Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4466 and GFF4467 are separated by 215 nucleotides GFF4467 and GFF4468 are separated by 156 nucleotides GFF4468 and GFF4469 are separated by 12 nucleotides
GFF4466 - Putative HTH-type transcriptional regulator ykgD, at 249,472 to 250,326
GFF4466
GFF4467 - Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4); Mercuric ion reductase (EC 1.16.1.1); PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase, at 250,542 to 251,867
GFF4467
GFF4468 - Putative periplasmic protein, at 252,024 to 252,260
GFF4468
GFF4469 - Putative inner membrane protein, at 252,273 to 252,833
GFF4469
Position (kb) 250
251
252 Strain fitness (log2 ratio) -2
-1
0
1
2 at 249.566 kb on + strand, within GFF4466 at 249.670 kb on - strand, within GFF4466 at 249.868 kb on - strand, within GFF4466 at 249.895 kb on + strand, within GFF4466 at 250.069 kb on - strand, within GFF4466 at 250.069 kb on - strand, within GFF4466 at 250.144 kb on - strand, within GFF4466 at 250.296 kb on + strand at 250.315 kb on - strand at 250.498 kb on - strand at 250.565 kb on + strand at 250.653 kb on - strand at 250.653 kb on - strand at 250.653 kb on - strand at 250.957 kb on + strand, within GFF4467 at 251.046 kb on - strand, within GFF4467 at 251.097 kb on - strand, within GFF4467 at 251.097 kb on - strand, within GFF4467 at 251.237 kb on - strand, within GFF4467 at 251.317 kb on + strand, within GFF4467 at 251.318 kb on - strand, within GFF4467 at 251.376 kb on + strand, within GFF4467 at 251.400 kb on - strand, within GFF4467 at 251.439 kb on - strand, within GFF4467 at 251.470 kb on + strand, within GFF4467 at 251.528 kb on + strand, within GFF4467 at 251.697 kb on - strand, within GFF4467 at 251.822 kb on + strand at 251.984 kb on - strand at 251.986 kb on + strand at 252.112 kb on - strand, within GFF4468 at 252.399 kb on - strand, within GFF4469 at 252.525 kb on - strand, within GFF4469 at 252.528 kb on - strand, within GFF4469 at 252.528 kb on - strand, within GFF4469 at 252.584 kb on - strand, within GFF4469 at 252.610 kb on + strand, within GFF4469 at 252.714 kb on + strand, within GFF4469 at 252.754 kb on + strand, within GFF4469 at 252.767 kb on - strand, within GFF4469
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 249,566 + GFF4466 0.11 +0.7 249,670 - GFF4466 0.23 +0.8 249,868 - GFF4466 0.46 -1.1 249,895 + GFF4466 0.49 +0.2 250,069 - GFF4466 0.70 -0.5 250,069 - GFF4466 0.70 +1.2 250,144 - GFF4466 0.79 -0.1 250,296 + -1.6 250,315 - -0.2 250,498 - -0.6 250,565 + -1.4 250,653 - +0.4 250,653 - -1.2 250,653 - +1.8 250,957 + GFF4467 0.31 +0.7 251,046 - GFF4467 0.38 +0.4 251,097 - GFF4467 0.42 +0.2 251,097 - GFF4467 0.42 -0.5 251,237 - GFF4467 0.52 +0.6 251,317 + GFF4467 0.58 +0.5 251,318 - GFF4467 0.59 -0.1 251,376 + GFF4467 0.63 -1.9 251,400 - GFF4467 0.65 +0.1 251,439 - GFF4467 0.68 -0.1 251,470 + GFF4467 0.70 -0.2 251,528 + GFF4467 0.74 +0.2 251,697 - GFF4467 0.87 +0.7 251,822 + -0.4 251,984 - -0.2 251,986 + -0.4 252,112 - GFF4468 0.37 +0.1 252,399 - GFF4469 0.22 -1.4 252,525 - GFF4469 0.45 +0.0 252,528 - GFF4469 0.45 +0.2 252,528 - GFF4469 0.45 +0.4 252,584 - GFF4469 0.55 -0.2 252,610 + GFF4469 0.60 +0.0 252,714 + GFF4469 0.79 -0.1 252,754 + GFF4469 0.86 -0.5 252,767 - GFF4469 0.88 +0.5
Or see this region's nucleotide sequence