Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4456 and GFF4457 are separated by 164 nucleotides GFF4457 and GFF4458 are separated by 22 nucleotides GFF4458 and GFF4657 are separated by 62 nucleotides GFF4657 and GFF4459 are separated by 564 nucleotides
GFF4456 - Putative diguanylate cyclase/phosphodiesterase domain 0, at 242,447 to 242,755
GFF4456
GFF4457 - Fimbriae W protein, at 242,920 to 243,516
GFF4457
GFF4458 - FIG01046307: hypothetical protein, at 243,539 to 243,709
GFF4458
GFF4657 - tRNA-Arg-TCT, at 243,772 to 243,848
GFF4657
GFF4459 - Mobile element protein, at 244,413 to 244,631
GFF4459
Position (kb) 243
244 Strain fitness (log2 ratio) -2
-1
0
1 at 242.581 kb on + strand, within GFF4456 at 243.288 kb on + strand, within GFF4457 at 243.328 kb on - strand, within GFF4457 at 243.398 kb on - strand, within GFF4457 at 243.398 kb on - strand, within GFF4457 at 243.498 kb on + strand at 243.507 kb on + strand at 243.982 kb on + strand at 243.983 kb on - strand at 244.044 kb on - strand at 244.106 kb on - strand at 244.106 kb on - strand at 244.291 kb on + strand at 244.330 kb on + strand at 244.385 kb on + strand at 244.387 kb on + strand at 244.530 kb on - strand, within GFF4459 at 244.530 kb on - strand, within GFF4459 at 244.533 kb on - strand, within GFF4459 at 244.587 kb on + strand, within GFF4459 at 244.646 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 242,581 + GFF4456 0.43 -1.5 243,288 + GFF4457 0.62 +0.9 243,328 - GFF4457 0.68 +0.5 243,398 - GFF4457 0.80 +0.3 243,398 - GFF4457 0.80 +1.0 243,498 + -0.1 243,507 + -0.2 243,982 + -2.5 243,983 - +1.3 244,044 - -0.2 244,106 - +1.0 244,106 - +1.2 244,291 + -1.7 244,330 + +0.2 244,385 + +0.2 244,387 + -0.5 244,530 - GFF4459 0.53 -0.2 244,530 - GFF4459 0.53 +0.4 244,533 - GFF4459 0.55 -0.2 244,587 + GFF4459 0.79 -0.4 244,646 - -0.3
Or see this region's nucleotide sequence