Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4412 and GFF4413 overlap by 30 nucleotides GFF4413 and GFF4414 overlap by 4 nucleotides GFF4414 and GFF4415 are separated by 185 nucleotides
GFF4412 - Arylesterase precursor (EC 3.1.1.2), at 196,068 to 196,682
GFF4412
GFF4413 - Uncharacterized metabolite ABC transporter in Enterobacteriaceae, ATP-binding protein EC-YbbA, at 196,653 to 197,339
GFF4413
GFF4414 - FIG00809136: hypothetical protein, at 197,336 to 199,750
GFF4414
GFF4415 - Putative outer membrane protein, at 199,936 to 201,069
GFF4415
Position (kb) 197
198
199
200 Strain fitness (log2 ratio) -2
-1
0
1 at 196.345 kb on - strand, within GFF4412 at 196.380 kb on + strand, within GFF4412 at 196.472 kb on + strand, within GFF4412 at 196.482 kb on + strand, within GFF4412 at 196.526 kb on + strand, within GFF4412 at 196.527 kb on - strand, within GFF4412 at 196.593 kb on + strand, within GFF4412 at 196.950 kb on - strand, within GFF4413 at 196.950 kb on - strand, within GFF4413 at 197.324 kb on - strand at 197.340 kb on + strand at 197.341 kb on - strand at 197.341 kb on - strand at 197.485 kb on + strand at 197.552 kb on - strand at 197.640 kb on + strand, within GFF4414 at 197.641 kb on - strand, within GFF4414 at 197.645 kb on - strand, within GFF4414 at 197.645 kb on - strand, within GFF4414 at 197.867 kb on - strand, within GFF4414 at 198.205 kb on + strand, within GFF4414 at 198.208 kb on + strand, within GFF4414 at 198.538 kb on + strand, within GFF4414 at 198.539 kb on - strand, within GFF4414 at 198.539 kb on - strand, within GFF4414 at 198.613 kb on + strand, within GFF4414 at 198.728 kb on - strand, within GFF4414 at 198.809 kb on + strand, within GFF4414 at 198.823 kb on + strand, within GFF4414 at 199.107 kb on + strand, within GFF4414 at 199.135 kb on + strand, within GFF4414 at 199.419 kb on + strand, within GFF4414 at 199.434 kb on + strand, within GFF4414 at 199.434 kb on + strand, within GFF4414 at 199.485 kb on + strand, within GFF4414 at 199.486 kb on - strand, within GFF4414 at 199.728 kb on + strand at 199.916 kb on + strand at 200.137 kb on - strand, within GFF4415 at 200.219 kb on - strand, within GFF4415 at 200.221 kb on + strand, within GFF4415 at 200.222 kb on - strand, within GFF4415 at 200.289 kb on + strand, within GFF4415 at 200.309 kb on - strand, within GFF4415 at 200.309 kb on - strand, within GFF4415 at 200.369 kb on - strand, within GFF4415 at 200.518 kb on + strand, within GFF4415 at 200.545 kb on + strand, within GFF4415 at 200.648 kb on - strand, within GFF4415 at 200.648 kb on - strand, within GFF4415 at 200.698 kb on - strand, within GFF4415
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 196,345 - GFF4412 0.45 +0.1 196,380 + GFF4412 0.51 +1.3 196,472 + GFF4412 0.66 -1.2 196,482 + GFF4412 0.67 -1.8 196,526 + GFF4412 0.74 +0.4 196,527 - GFF4412 0.75 +0.0 196,593 + GFF4412 0.85 -2.2 196,950 - GFF4413 0.43 +1.0 196,950 - GFF4413 0.43 -1.6 197,324 - -0.3 197,340 + -0.7 197,341 - -0.8 197,341 - -1.3 197,485 + -0.5 197,552 - -1.5 197,640 + GFF4414 0.13 +0.3 197,641 - GFF4414 0.13 +0.6 197,645 - GFF4414 0.13 -0.1 197,645 - GFF4414 0.13 -0.7 197,867 - GFF4414 0.22 -1.2 198,205 + GFF4414 0.36 -1.3 198,208 + GFF4414 0.36 +0.3 198,538 + GFF4414 0.50 +0.0 198,539 - GFF4414 0.50 -0.3 198,539 - GFF4414 0.50 +0.2 198,613 + GFF4414 0.53 +1.1 198,728 - GFF4414 0.58 +0.4 198,809 + GFF4414 0.61 -0.1 198,823 + GFF4414 0.62 -1.5 199,107 + GFF4414 0.73 -1.2 199,135 + GFF4414 0.74 -2.0 199,419 + GFF4414 0.86 -0.9 199,434 + GFF4414 0.87 -0.2 199,434 + GFF4414 0.87 +0.6 199,485 + GFF4414 0.89 +0.4 199,486 - GFF4414 0.89 +0.6 199,728 + +0.6 199,916 + +0.9 200,137 - GFF4415 0.18 -0.7 200,219 - GFF4415 0.25 -0.7 200,221 + GFF4415 0.25 -0.5 200,222 - GFF4415 0.25 -0.3 200,289 + GFF4415 0.31 +0.2 200,309 - GFF4415 0.33 -0.3 200,309 - GFF4415 0.33 +0.1 200,369 - GFF4415 0.38 -0.8 200,518 + GFF4415 0.51 +0.6 200,545 + GFF4415 0.54 +0.0 200,648 - GFF4415 0.63 -0.2 200,648 - GFF4415 0.63 +0.1 200,698 - GFF4415 0.67 +0.3
Or see this region's nucleotide sequence