Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4357 and GFF4358 are separated by 149 nucleotides GFF4358 and GFF4359 are separated by 103 nucleotides GFF4359 and GFF4360 are separated by 105 nucleotides GFF4360 and GFF4361 are separated by 64 nucleotides
GFF4357 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 138,880 to 140,751
GFF4357
GFF4358 - FIG01200701: possible membrane protein, at 140,901 to 141,275
GFF4358
GFF4359 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 141,379 to 141,777
GFF4359
GFF4360 - Queuosine Biosynthesis QueC ATPase, at 141,883 to 142,578
GFF4360
GFF4361 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 142,643 to 144,343
GFF4361
Position (kb) 141
142 Strain fitness (log2 ratio) -2
-1
0
1
2 at 140.915 kb on + strand at 141.183 kb on + strand, within GFF4358 at 141.251 kb on + strand at 141.273 kb on + strand at 141.274 kb on - strand at 141.336 kb on + strand at 141.337 kb on - strand at 141.347 kb on + strand at 141.352 kb on - strand at 141.354 kb on - strand at 141.354 kb on - strand at 141.365 kb on - strand at 141.551 kb on + strand, within GFF4359 at 141.584 kb on + strand, within GFF4359 at 141.585 kb on - strand, within GFF4359 at 141.632 kb on + strand, within GFF4359 at 141.821 kb on - strand at 141.836 kb on + strand at 141.890 kb on - strand at 142.003 kb on - strand, within GFF4360 at 142.087 kb on - strand, within GFF4360 at 142.099 kb on + strand, within GFF4360 at 142.506 kb on - strand, within GFF4360 at 142.559 kb on - strand at 142.579 kb on - strand at 142.579 kb on - strand at 142.579 kb on - strand at 142.582 kb on + strand at 142.750 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 140,915 + -0.1 141,183 + GFF4358 0.75 -0.3 141,251 + +0.4 141,273 + -2.1 141,274 - +1.8 141,336 + +0.2 141,337 - -1.7 141,347 + -1.9 141,352 - -0.9 141,354 - -0.3 141,354 - -0.7 141,365 - -0.4 141,551 + GFF4359 0.43 -0.4 141,584 + GFF4359 0.51 -0.1 141,585 - GFF4359 0.52 -0.0 141,632 + GFF4359 0.63 -1.6 141,821 - -0.2 141,836 + +0.9 141,890 - -0.9 142,003 - GFF4360 0.17 +0.5 142,087 - GFF4360 0.29 +2.5 142,099 + GFF4360 0.31 +0.9 142,506 - GFF4360 0.90 -0.6 142,559 - +0.2 142,579 - +0.6 142,579 - +0.2 142,579 - -0.7 142,582 + +0.2 142,750 + +0.5
Or see this region's nucleotide sequence