Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4325

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF4324 and GFF4325 are separated by 23 nucleotidesGFF4325 and GFF4326 are separated by 0 nucleotidesGFF4326 and GFF4327 are separated by 209 nucleotides GFF4324 - 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7), at 104,842 to 106,704 GFF4324 GFF4325 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 106,728 to 107,627 GFF4325 GFF4326 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 107,628 to 107,870 GFF4326 GFF4327 - tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis, at 108,080 to 109,528 GFF4327 Position (kb) 106 107 108Strain fitness (log2 ratio) -1 0 1 2 3 4at 107.901 kb on - strandat 107.901 kb on - strandat 107.985 kb on + strandat 107.988 kb on - strandat 108.012 kb on - strandat 108.012 kb on - strandat 108.028 kb on - strandat 108.031 kb on - strandat 108.031 kb on - strandat 108.051 kb on - strandat 108.054 kb on + strandat 108.055 kb on - strandat 108.055 kb on - strandat 108.180 kb on + strandat 108.304 kb on + strand, within GFF4327at 108.575 kb on + strand, within GFF4327at 108.576 kb on - strand, within GFF4327at 108.583 kb on + strand, within GFF4327

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
remove
107,901 - -0.1
107,901 - -0.4
107,985 + +0.2
107,988 - +1.3
108,012 - +3.8
108,012 - +0.3
108,028 - +2.5
108,031 - +1.6
108,031 - +2.3
108,051 - +1.2
108,054 + -0.1
108,055 - +1.2
108,055 - +1.3
108,180 + +2.7
108,304 + GFF4327 0.15 -1.5
108,575 + GFF4327 0.34 +0.8
108,576 - GFF4327 0.34 +4.4
108,583 + GFF4327 0.35 +2.4

Or see this region's nucleotide sequence