Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4324 and GFF4325 are separated by 23 nucleotides GFF4325 and GFF4326 are separated by 0 nucleotides GFF4326 and GFF4327 are separated by 209 nucleotides
GFF4324 - 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7), at 104,842 to 106,704
GFF4324
GFF4325 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 106,728 to 107,627
GFF4325
GFF4326 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 107,628 to 107,870
GFF4326
GFF4327 - tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis, at 108,080 to 109,528
GFF4327
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 107.901 kb on - strand at 107.901 kb on - strand at 107.985 kb on + strand at 107.988 kb on - strand at 108.012 kb on - strand at 108.012 kb on - strand at 108.028 kb on - strand at 108.031 kb on - strand at 108.031 kb on - strand at 108.051 kb on - strand at 108.054 kb on + strand at 108.055 kb on - strand at 108.055 kb on - strand at 108.180 kb on + strand at 108.304 kb on + strand, within GFF4327 at 108.575 kb on + strand, within GFF4327 at 108.576 kb on - strand, within GFF4327 at 108.583 kb on + strand, within GFF4327
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 107,901 - -0.1 107,901 - -0.4 107,985 + +0.2 107,988 - +1.3 108,012 - +3.8 108,012 - +0.3 108,028 - +2.5 108,031 - +1.6 108,031 - +2.3 108,051 - +1.2 108,054 + -0.1 108,055 - +1.2 108,055 - +1.3 108,180 + +2.7 108,304 + GFF4327 0.15 -1.5 108,575 + GFF4327 0.34 +0.8 108,576 - GFF4327 0.34 +4.4 108,583 + GFF4327 0.35 +2.4
Or see this region's nucleotide sequence