Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4324

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4323 and GFF4324 are separated by 56 nucleotidesGFF4324 and GFF4325 are separated by 23 nucleotidesGFF4325 and GFF4326 are separated by 0 nucleotides GFF4323 - FIG00553873: hypothetical protein, at 103,811 to 104,785 GFF4323 GFF4324 - 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7), at 104,842 to 106,704 GFF4324 GFF4325 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 106,728 to 107,627 GFF4325 GFF4326 - Exodeoxyribonuclease VII small subunit (EC 3.1.11.6), at 107,628 to 107,870 GFF4326 Position (kb) 104 105 106 107Strain fitness (log2 ratio) -1 0 1at 103.911 kb on - strand, within GFF4323at 104.053 kb on + strand, within GFF4323at 104.129 kb on + strand, within GFF4323at 104.328 kb on + strand, within GFF4323at 104.726 kb on - strandat 104.778 kb on + strandat 104.779 kb on - strandat 104.822 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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103,911 - GFF4323 0.10 -0.2
104,053 + GFF4323 0.25 -0.3
104,129 + GFF4323 0.33 +0.6
104,328 + GFF4323 0.53 +0.1
104,726 - -0.4
104,778 + +0.5
104,779 - -0.0
104,822 + +0.5

Or see this region's nucleotide sequence