Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4299 and GFF4300 are separated by 14 nucleotides GFF4300 and GFF4301 are separated by 161 nucleotides
GFF4299 - Branched-chain amino acid transport system carrier protein, at 83,470 to 84,789
GFF4299
GFF4300 - Proline-specific permease proY, at 84,804 to 86,234
GFF4300
GFF4301 - Maltodextrin glucosidase (EC 3.2.1.20), at 86,396 to 88,213
GFF4301
Position (kb) 84
85
86
87 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 83.842 kb on + strand, within GFF4299 at 84.010 kb on + strand, within GFF4299 at 84.288 kb on + strand, within GFF4299 at 84.288 kb on + strand, within GFF4299 at 84.289 kb on - strand, within GFF4299 at 84.289 kb on - strand, within GFF4299 at 84.394 kb on + strand, within GFF4299 at 84.564 kb on + strand, within GFF4299 at 84.802 kb on - strand at 84.802 kb on - strand at 84.802 kb on - strand at 84.802 kb on - strand at 84.807 kb on + strand at 84.808 kb on - strand at 84.879 kb on - strand at 84.883 kb on + strand at 84.884 kb on - strand at 84.926 kb on - strand at 85.005 kb on + strand, within GFF4300 at 85.008 kb on - strand, within GFF4300 at 85.031 kb on + strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.032 kb on - strand, within GFF4300 at 85.147 kb on - strand, within GFF4300 at 85.244 kb on - strand, within GFF4300 at 85.343 kb on + strand, within GFF4300 at 85.343 kb on + strand, within GFF4300 at 85.344 kb on - strand, within GFF4300 at 85.346 kb on + strand, within GFF4300 at 85.346 kb on + strand, within GFF4300 at 85.572 kb on - strand, within GFF4300 at 85.572 kb on - strand, within GFF4300 at 85.625 kb on + strand, within GFF4300 at 85.632 kb on - strand, within GFF4300 at 85.784 kb on + strand, within GFF4300 at 85.911 kb on + strand, within GFF4300 at 85.912 kb on - strand, within GFF4300 at 85.970 kb on - strand, within GFF4300 at 85.982 kb on + strand, within GFF4300 at 86.152 kb on - strand at 86.198 kb on + strand at 86.341 kb on + strand at 86.638 kb on + strand, within GFF4301 at 86.638 kb on + strand, within GFF4301 at 86.937 kb on + strand, within GFF4301 at 86.986 kb on - strand, within GFF4301 at 86.986 kb on - strand, within GFF4301 at 87.000 kb on + strand, within GFF4301 at 87.022 kb on + strand, within GFF4301 at 87.022 kb on + strand, within GFF4301 at 87.023 kb on - strand, within GFF4301
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 83,842 + GFF4299 0.28 +1.3 84,010 + GFF4299 0.41 +1.1 84,288 + GFF4299 0.62 +1.0 84,288 + GFF4299 0.62 +0.9 84,289 - GFF4299 0.62 +1.1 84,289 - GFF4299 0.62 +1.6 84,394 + GFF4299 0.70 +0.8 84,564 + GFF4299 0.83 +0.3 84,802 - +0.2 84,802 - +1.3 84,802 - -0.1 84,802 - -0.3 84,807 + +0.6 84,808 - -0.8 84,879 - -0.2 84,883 + -1.7 84,884 - -2.3 84,926 - -0.1 85,005 + GFF4300 0.14 -0.2 85,008 - GFF4300 0.14 -0.6 85,031 + GFF4300 0.16 -0.4 85,032 - GFF4300 0.16 -0.9 85,032 - GFF4300 0.16 +0.1 85,032 - GFF4300 0.16 +0.3 85,032 - GFF4300 0.16 -1.0 85,147 - GFF4300 0.24 -1.2 85,244 - GFF4300 0.31 -0.1 85,343 + GFF4300 0.38 -1.7 85,343 + GFF4300 0.38 -0.2 85,344 - GFF4300 0.38 -0.2 85,346 + GFF4300 0.38 +0.8 85,346 + GFF4300 0.38 -0.2 85,572 - GFF4300 0.54 -0.9 85,572 - GFF4300 0.54 -0.9 85,625 + GFF4300 0.57 -1.3 85,632 - GFF4300 0.58 -0.9 85,784 + GFF4300 0.68 -3.8 85,911 + GFF4300 0.77 +0.0 85,912 - GFF4300 0.77 +0.0 85,970 - GFF4300 0.81 +0.5 85,982 + GFF4300 0.82 -1.0 86,152 - -1.0 86,198 + -0.5 86,341 + -0.4 86,638 + GFF4301 0.13 -1.5 86,638 + GFF4301 0.13 -0.7 86,937 + GFF4301 0.30 +0.9 86,986 - GFF4301 0.32 +0.3 86,986 - GFF4301 0.32 +0.1 87,000 + GFF4301 0.33 -1.5 87,022 + GFF4301 0.34 +0.3 87,022 + GFF4301 0.34 -0.7 87,023 - GFF4301 0.34 +0.5
Or see this region's nucleotide sequence