Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4241

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4238 and GFF4239 overlap by 28 nucleotidesGFF4239 and GFF4240 are separated by 121 nucleotidesGFF4240 and GFF4241 are separated by 141 nucleotidesGFF4241 and GFF4242 are separated by 143 nucleotidesGFF4242 and GFF4243 are separated by 55 nucleotides GFF4238 - Possible transmembrane regulator, at 18,995 to 19,738 GFF4238 GFF4239 - Putative inner membrane protein, at 19,711 to 20,199 GFF4239 GFF4240 - hypothetical protein, at 20,321 to 20,458 GFF4240 GFF4241 - Attachment invasion locus protein precursor, at 20,600 to 21,124 GFF4241 GFF4242 - Possible transcriptionl regulator, at 21,268 to 21,909 GFF4242 GFF4243 - Probable secreted protein, at 21,965 to 22,144 GFF4243 Position (kb) 20 21 22Strain fitness (log2 ratio) -2 -1 0 1 2at 20.087 kb on + strand, within GFF4239at 20.131 kb on - strand, within GFF4239at 20.197 kb on + strandat 21.200 kb on - strandat 21.806 kb on - strand, within GFF4242at 21.859 kb on - strandat 21.984 kb on + strand, within GFF4243

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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20,087 + GFF4239 0.77 -1.8
20,131 - GFF4239 0.86 +0.8
20,197 + +0.4
21,200 - -0.1
21,806 - GFF4242 0.84 +1.9
21,859 - +0.5
21,984 + GFF4243 0.11 -0.6

Or see this region's nucleotide sequence