Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4150 and GFF4151 are separated by 1 nucleotides GFF4151 and GFF4152 are separated by 13 nucleotides GFF4152 and GFF4153 are separated by 600 nucleotides
GFF4150 - putative periplasmic protein, at 31,135 to 31,773
GFF4150
GFF4151 - integrase-like protein, at 31,775 to 31,942
GFF4151
GFF4152 - Phage integrase, Phage P4-associated, at 31,956 to 33,020
GFF4152
GFF4153 - Type I secretion system, membrane fusion protein LapC, at 33,621 to 34,811
GFF4153
Position (kb)
31
32
33
34 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 30.990 kb on + strand at 30.991 kb on - strand at 31.043 kb on + strand at 31.043 kb on + strand at 31.072 kb on - strand at 31.238 kb on - strand, within GFF4150 at 31.238 kb on - strand, within GFF4150 at 31.477 kb on - strand, within GFF4150 at 31.515 kb on + strand, within GFF4150 at 31.550 kb on + strand, within GFF4150 at 31.627 kb on - strand, within GFF4150 at 31.631 kb on + strand, within GFF4150 at 31.729 kb on - strand at 32.069 kb on + strand, within GFF4152 at 32.069 kb on + strand, within GFF4152 at 32.069 kb on + strand, within GFF4152 at 32.287 kb on - strand, within GFF4152 at 32.481 kb on - strand, within GFF4152 at 32.736 kb on + strand, within GFF4152 at 32.834 kb on - strand, within GFF4152 at 32.881 kb on + strand, within GFF4152 at 32.946 kb on + strand at 32.947 kb on - strand at 33.242 kb on - strand at 33.250 kb on - strand at 33.261 kb on - strand at 33.304 kb on - strand at 33.562 kb on - strand at 33.562 kb on - strand at 33.565 kb on + strand at 33.608 kb on + strand at 33.623 kb on - strand at 33.705 kb on + strand at 33.706 kb on - strand at 33.759 kb on + strand, within GFF4153 at 33.871 kb on + strand, within GFF4153 at 33.927 kb on - strand, within GFF4153
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 30,990 + -1.2 30,991 - -1.2 31,043 + +0.4 31,043 + -0.2 31,072 - -0.6 31,238 - GFF4150 0.16 -0.0 31,238 - GFF4150 0.16 +0.0 31,477 - GFF4150 0.54 +1.1 31,515 + GFF4150 0.59 +0.2 31,550 + GFF4150 0.65 -0.2 31,627 - GFF4150 0.77 -0.1 31,631 + GFF4150 0.78 +2.6 31,729 - -1.3 32,069 + GFF4152 0.11 -3.0 32,069 + GFF4152 0.11 +0.4 32,069 + GFF4152 0.11 -1.3 32,287 - GFF4152 0.31 -0.3 32,481 - GFF4152 0.49 -1.1 32,736 + GFF4152 0.73 -1.9 32,834 - GFF4152 0.82 +0.1 32,881 + GFF4152 0.87 -0.5 32,946 + -1.0 32,947 - +0.2 33,242 - +5.6 33,250 - +5.0 33,261 - +5.1 33,304 - +5.3 33,562 - +3.6 33,562 - +3.8 33,565 + +6.1 33,608 + -1.1 33,623 - +0.8 33,705 + -0.2 33,706 - +0.2 33,759 + GFF4153 0.12 -0.3 33,871 + GFF4153 0.21 -0.3 33,927 - GFF4153 0.26 +1.2
Or see this region's nucleotide sequence