Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4130 and GFF4131 are separated by 222 nucleotides
GFF4130 - putative dipeptide/oligopeptide/nickel ABC-type transport system periplasmic component, at 11,867 to 13,546
GFF4130
GFF4131 - PTS system, glucose-specific IIC component (EC 2.7.1.69) / PTS system, glucose-specific IIB component (EC 2.7.1.69) / PTS system, glucose-specific IIA component (EC 2.7.1.69), at 13,769 to 15,310
GFF4131
Position (kb)
11
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 10.868 kb on - strand at 10.868 kb on - strand at 11.008 kb on + strand at 11.016 kb on + strand at 11.131 kb on - strand at 11.181 kb on - strand at 11.332 kb on + strand at 11.584 kb on - strand at 11.625 kb on + strand at 11.666 kb on + strand at 11.671 kb on + strand at 11.687 kb on - strand at 11.832 kb on + strand at 11.926 kb on + strand at 11.980 kb on - strand at 11.980 kb on - strand at 12.059 kb on + strand, within GFF4130 at 12.248 kb on - strand, within GFF4130 at 12.398 kb on - strand, within GFF4130 at 12.477 kb on + strand, within GFF4130 at 12.478 kb on - strand, within GFF4130 at 12.484 kb on + strand, within GFF4130 at 12.484 kb on + strand, within GFF4130 at 12.713 kb on - strand, within GFF4130 at 12.774 kb on + strand, within GFF4130 at 12.845 kb on + strand, within GFF4130 at 13.170 kb on + strand, within GFF4130 at 13.201 kb on + strand, within GFF4130 at 13.245 kb on + strand, within GFF4130 at 13.541 kb on - strand at 13.546 kb on + strand at 13.745 kb on + strand at 13.797 kb on + strand at 13.798 kb on - strand at 14.072 kb on - strand, within GFF4131 at 14.136 kb on - strand, within GFF4131 at 14.219 kb on - strand, within GFF4131 at 14.536 kb on + strand, within GFF4131
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 10,868 - +1.1 10,868 - -0.2 11,008 + +1.1 11,016 + +0.1 11,131 - +0.3 11,181 - +0.1 11,332 + -1.4 11,584 - -1.8 11,625 + +0.2 11,666 + +1.0 11,671 + -0.3 11,687 - -0.6 11,832 + -0.2 11,926 + -0.5 11,980 - +0.2 11,980 - +0.4 12,059 + GFF4130 0.11 -0.0 12,248 - GFF4130 0.23 +0.3 12,398 - GFF4130 0.32 -0.6 12,477 + GFF4130 0.36 +0.1 12,478 - GFF4130 0.36 -0.1 12,484 + GFF4130 0.37 -1.0 12,484 + GFF4130 0.37 +0.5 12,713 - GFF4130 0.50 -0.8 12,774 + GFF4130 0.54 -2.1 12,845 + GFF4130 0.58 -3.3 13,170 + GFF4130 0.78 -0.2 13,201 + GFF4130 0.79 -0.7 13,245 + GFF4130 0.82 -0.2 13,541 - +0.8 13,546 + +0.5 13,745 + -0.7 13,797 + +0.3 13,798 - -0.2 14,072 - GFF4131 0.20 -2.6 14,136 - GFF4131 0.24 -1.8 14,219 - GFF4131 0.29 -0.0 14,536 + GFF4131 0.50 +0.1
Or see this region's nucleotide sequence