Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF403

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF402 and GFF403 are separated by 77 nucleotidesGFF403 and GFF404 are separated by 82 nucleotides GFF402 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 117,042 to 118,061 GFF402 GFF403 - Serine acetyltransferase (EC 2.3.1.30), at 118,139 to 118,960 GFF403 GFF404 - D-glucarate permease, at 119,043 to 120,353 GFF404 Position (kb) 118 119Strain fitness (log2 ratio) -2 -1 0 1at 117.580 kb on - strand, within GFF402at 118.933 kb on + strandat 118.970 kb on + strandat 119.115 kb on - strandat 119.135 kb on - strandat 119.225 kb on + strand, within GFF404at 119.225 kb on + strand, within GFF404at 119.229 kb on + strand, within GFF404at 119.359 kb on + strand, within GFF404at 119.393 kb on + strand, within GFF404at 119.403 kb on - strand, within GFF404at 119.433 kb on - strand, within GFF404at 119.560 kb on + strand, within GFF404at 119.595 kb on - strand, within GFF404at 119.693 kb on + strand, within GFF404

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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117,580 - GFF402 0.53 +0.5
118,933 + +1.1
118,970 + -2.3
119,115 - -1.3
119,135 - -0.1
119,225 + GFF404 0.14 +0.7
119,225 + GFF404 0.14 -0.0
119,229 + GFF404 0.14 +1.1
119,359 + GFF404 0.24 +0.3
119,393 + GFF404 0.27 -0.0
119,403 - GFF404 0.27 +0.7
119,433 - GFF404 0.30 -1.7
119,560 + GFF404 0.39 -1.4
119,595 - GFF404 0.42 +0.0
119,693 + GFF404 0.50 +1.0

Or see this region's nucleotide sequence