Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3893

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3891 and GFF3892 overlap by 4 nucleotidesGFF3892 and GFF3893 are separated by 4 nucleotidesGFF3893 and GFF3894 are separated by 26 nucleotides GFF3891 - CDP-6-deoxy-delta-3,4-glucoseen reductase-like, at 169,741 to 170,733 GFF3891 GFF3892 - Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33), at 170,730 to 171,503 GFF3892 GFF3893 - Similar to CDP-glucose 4,6-dehydratase (EC 4.2.1.45), at 171,508 to 172,587 GFF3893 GFF3894 - CDP-4-dehydro-6-deoxy-D-glucose 3-dehydratase (EC 4.2.1.-), at 172,614 to 173,927 GFF3894 Position (kb) 171 172 173Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 170.606 kb on + strand, within GFF3891at 171.733 kb on - strand, within GFF3893at 172.566 kb on + strandat 172.579 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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170,606 + GFF3891 0.87 +0.0
171,733 - GFF3893 0.21 -0.3
172,566 + -4.0
172,579 - +1.3

Or see this region's nucleotide sequence