Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3845 and GFF3846 are separated by 489 nucleotides GFF3846 and GFF3847 are separated by 115 nucleotides
GFF3845 - putative cytoplasmic protein, at 112,310 to 113,317
GFF3845
GFF3846 - Putative protease, at 113,807 to 115,168
GFF3846
GFF3847 - Putative inner membrane protein, at 115,284 to 117,395
GFF3847
Position (kb)
113
114
115
116 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 113.228 kb on + strand at 113.552 kb on - strand at 113.552 kb on - strand at 113.629 kb on + strand at 113.696 kb on - strand at 113.709 kb on + strand at 113.812 kb on + strand at 113.812 kb on + strand at 113.906 kb on - strand at 114.209 kb on - strand, within GFF3846 at 114.282 kb on + strand, within GFF3846 at 114.384 kb on + strand, within GFF3846 at 114.384 kb on + strand, within GFF3846 at 114.384 kb on + strand, within GFF3846 at 114.385 kb on - strand, within GFF3846 at 114.444 kb on - strand, within GFF3846 at 114.566 kb on - strand, within GFF3846 at 114.567 kb on + strand, within GFF3846 at 114.812 kb on - strand, within GFF3846 at 114.866 kb on - strand, within GFF3846 at 115.052 kb on - strand at 115.052 kb on - strand at 115.068 kb on + strand at 115.087 kb on - strand at 115.285 kb on + strand at 115.285 kb on + strand at 115.286 kb on - strand at 115.339 kb on + strand at 115.339 kb on + strand at 115.466 kb on + strand at 115.467 kb on - strand at 115.569 kb on - strand, within GFF3847 at 115.581 kb on - strand, within GFF3847 at 115.631 kb on + strand, within GFF3847 at 115.754 kb on + strand, within GFF3847 at 115.896 kb on + strand, within GFF3847 at 115.899 kb on + strand, within GFF3847 at 115.919 kb on + strand, within GFF3847 at 115.958 kb on + strand, within GFF3847 at 116.045 kb on + strand, within GFF3847 at 116.045 kb on + strand, within GFF3847 at 116.052 kb on - strand, within GFF3847 at 116.053 kb on + strand, within GFF3847
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 113,228 + -0.4 113,552 - +0.7 113,552 - +1.7 113,629 + +0.6 113,696 - -0.4 113,709 + +0.7 113,812 + -0.6 113,812 + -1.3 113,906 - -2.0 114,209 - GFF3846 0.30 -1.4 114,282 + GFF3846 0.35 -0.5 114,384 + GFF3846 0.42 +0.3 114,384 + GFF3846 0.42 -1.1 114,384 + GFF3846 0.42 -1.3 114,385 - GFF3846 0.42 -0.9 114,444 - GFF3846 0.47 -1.4 114,566 - GFF3846 0.56 +0.9 114,567 + GFF3846 0.56 +1.9 114,812 - GFF3846 0.74 -0.2 114,866 - GFF3846 0.78 -0.5 115,052 - -1.9 115,052 - -2.3 115,068 + -0.9 115,087 - +0.5 115,285 + +0.0 115,285 + +0.3 115,286 - +0.5 115,339 + +0.3 115,339 + -0.5 115,466 + +0.2 115,467 - -0.3 115,569 - GFF3847 0.13 -1.3 115,581 - GFF3847 0.14 -1.7 115,631 + GFF3847 0.16 +1.2 115,754 + GFF3847 0.22 +0.4 115,896 + GFF3847 0.29 -0.7 115,899 + GFF3847 0.29 -0.7 115,919 + GFF3847 0.30 +0.7 115,958 + GFF3847 0.32 -0.4 116,045 + GFF3847 0.36 -0.2 116,045 + GFF3847 0.36 +0.6 116,052 - GFF3847 0.36 -2.9 116,053 + GFF3847 0.36 +2.0
Or see this region's nucleotide sequence