Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3731

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3728 and GFF3729 are separated by 7 nucleotidesGFF3729 and GFF3730 are separated by 125 nucleotidesGFF3730 and GFF3731 are separated by 95 nucleotidesGFF3731 and GFF3732 are separated by 96 nucleotides GFF3728 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 31,456 to 31,812 GFF3728 GFF3729 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 31,820 to 32,107 GFF3729 GFF3730 - SSU ribosomal protein S12p (S23e), at 32,233 to 32,607 GFF3730 GFF3731 - SSU ribosomal protein S7p (S5e), at 32,703 to 33,173 GFF3731 GFF3732 - Translation elongation factor G, at 33,270 to 35,384 GFF3732 Position (kb) 32 33 34Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 32.122 kb on + strandat 32.189 kb on + strandat 32.189 kb on + strandat 32.192 kb on + strandat 32.192 kb on + strandat 33.212 kb on + strandat 33.235 kb on + strandat 33.235 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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32,122 + +0.3
32,189 + +4.5
32,189 + +4.1
32,192 + +5.1
32,192 + +4.3
33,212 + +3.4
33,235 + +1.9
33,235 + +1.5

Or see this region's nucleotide sequence