Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF369

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF367 and GFF368 are separated by 77 nucleotidesGFF368 and GFF369 are separated by 107 nucleotidesGFF369 and GFF370 overlap by 20 nucleotides GFF367 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 86,478 to 87,119 GFF367 GFF368 - Transcriptional regulator SlmA, TetR family, at 87,197 to 87,793 GFF368 GFF369 - 'Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23)' transl_table=11, at 87,901 to 88,356 GFF369 GFF370 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 88,337 to 89,560 GFF370 Position (kb) 87 88 89Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 86.997 kb on + strand, within GFF367at 87.005 kb on + strand, within GFF367at 87.006 kb on - strand, within GFF367at 87.006 kb on - strand, within GFF367at 87.006 kb on - strand, within GFF367at 87.006 kb on - strand, within GFF367at 87.006 kb on - strand, within GFF367at 87.151 kb on - strandat 87.151 kb on - strandat 87.290 kb on - strand, within GFF368at 87.343 kb on - strand, within GFF368at 87.375 kb on - strand, within GFF368at 87.844 kb on - strandat 87.875 kb on + strandat 87.875 kb on + strandat 87.902 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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86,997 + GFF367 0.81 +1.6
87,005 + GFF367 0.82 +0.5
87,006 - GFF367 0.82 -1.2
87,006 - GFF367 0.82 -0.7
87,006 - GFF367 0.82 +0.5
87,006 - GFF367 0.82 +1.5
87,006 - GFF367 0.82 +1.2
87,151 - +0.1
87,151 - +2.1
87,290 - GFF368 0.16 +0.2
87,343 - GFF368 0.24 +0.9
87,375 - GFF368 0.30 -0.4
87,844 - +0.2
87,875 + -3.4
87,875 + +0.3
87,902 + -0.9

Or see this region's nucleotide sequence