Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3598 and GFF3599 are separated by 56 nucleotides GFF3599 and GFF3600 are separated by 17 nucleotides GFF3600 and GFF3601 overlap by 4 nucleotides GFF3601 and GFF3602 overlap by 11 nucleotides
GFF3598 - Type III secretion protein SsaI, at 352,070 to 352,333
GFF3598
GFF3599 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 352,390 to 353,079
GFF3599
GFF3600 - FIG029138: Type III secretion protein, at 353,097 to 353,645
GFF3600
GFF3601 - Type III secretion protein SsaK, at 353,642 to 354,316
GFF3601
GFF3602 - Type III secretion cytoplasmic protein (YscL), at 354,306 to 355,298
GFF3602
Position (kb) 353
354 Strain fitness (log2 ratio) -1
0
1 at 352.349 kb on + strand at 352.366 kb on - strand at 352.494 kb on - strand, within GFF3599 at 352.608 kb on - strand, within GFF3599 at 352.990 kb on + strand, within GFF3599 at 353.467 kb on + strand, within GFF3600 at 353.937 kb on + strand, within GFF3601 at 354.003 kb on + strand, within GFF3601 at 354.053 kb on - strand, within GFF3601 at 354.277 kb on - strand at 354.387 kb on - strand at 354.403 kb on + strand at 354.644 kb on + strand, within GFF3602
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 352,349 + -0.0 352,366 - +1.6 352,494 - GFF3599 0.15 +0.2 352,608 - GFF3599 0.32 +1.7 352,990 + GFF3599 0.87 -0.2 353,467 + GFF3600 0.67 -0.6 353,937 + GFF3601 0.44 -1.1 354,003 + GFF3601 0.53 +1.4 354,053 - GFF3601 0.61 -1.2 354,277 - +1.1 354,387 - +0.9 354,403 + -0.6 354,644 + GFF3602 0.34 -1.3
Or see this region's nucleotide sequence