Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3500

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3498 and GFF3499 are separated by 93 nucleotidesGFF3499 and GFF3500 overlap by 48 nucleotidesGFF3500 and GFF3501 are separated by 131 nucleotidesGFF3501 and GFF3502 are separated by 300 nucleotides GFF3498 - Excinuclease cho (excinuclease ABC alternative C subunit), at 251,170 to 252,078 GFF3498 GFF3499 - NAD synthetase (EC 6.3.1.5), at 252,172 to 252,999 GFF3499 GFF3500 - hypothetical protein, at 252,952 to 253,086 GFF3500 GFF3501 - Osmotically inducible lipoprotein E precursor, at 253,218 to 253,559 GFF3501 GFF3502 - PTS system, cellobiose-specific IIB component (EC 2.7.1.69), at 253,860 to 254,180 GFF3502 Position (kb) 252 253 254Strain fitness (log2 ratio) -3 -2 -1 0 1at 252.085 kb on + strandat 252.168 kb on - strandat 252.168 kb on - strandat 252.168 kb on - strandat 252.173 kb on + strandat 253.190 kb on - strandat 253.265 kb on + strand, within GFF3501at 253.266 kb on - strand, within GFF3501at 253.266 kb on - strand, within GFF3501at 253.287 kb on + strand, within GFF3501at 253.288 kb on - strand, within GFF3501at 253.428 kb on + strand, within GFF3501at 253.465 kb on - strand, within GFF3501at 253.558 kb on - strandat 253.558 kb on - strandat 253.566 kb on + strandat 253.567 kb on - strandat 253.879 kb on - strandat 254.023 kb on + strand, within GFF3502

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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252,085 + +0.8
252,168 - -1.1
252,168 - -0.3
252,168 - -0.2
252,173 + +0.0
253,190 - -0.3
253,265 + GFF3501 0.14 +0.3
253,266 - GFF3501 0.14 -0.3
253,266 - GFF3501 0.14 -1.5
253,287 + GFF3501 0.20 +0.6
253,288 - GFF3501 0.20 -3.1
253,428 + GFF3501 0.61 +0.6
253,465 - GFF3501 0.72 -1.0
253,558 - -0.4
253,558 - +1.2
253,566 + +1.1
253,567 - +0.8
253,879 - +0.3
254,023 + GFF3502 0.51 +0.3

Or see this region's nucleotide sequence