Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3498 and GFF3499 are separated by 93 nucleotides GFF3499 and GFF3500 overlap by 48 nucleotides GFF3500 and GFF3501 are separated by 131 nucleotides GFF3501 and GFF3502 are separated by 300 nucleotides
GFF3498 - Excinuclease cho (excinuclease ABC alternative C subunit), at 251,170 to 252,078
GFF3498
GFF3499 - NAD synthetase (EC 6.3.1.5), at 252,172 to 252,999
GFF3499
GFF3500 - hypothetical protein, at 252,952 to 253,086
GFF3500
GFF3501 - Osmotically inducible lipoprotein E precursor, at 253,218 to 253,559
GFF3501
GFF3502 - PTS system, cellobiose-specific IIB component (EC 2.7.1.69), at 253,860 to 254,180
GFF3502
Position (kb)
252
253
254 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 252.085 kb on + strand at 252.168 kb on - strand at 252.168 kb on - strand at 252.168 kb on - strand at 252.173 kb on + strand at 253.190 kb on - strand at 253.265 kb on + strand, within GFF3501 at 253.266 kb on - strand, within GFF3501 at 253.266 kb on - strand, within GFF3501 at 253.287 kb on + strand, within GFF3501 at 253.288 kb on - strand, within GFF3501 at 253.428 kb on + strand, within GFF3501 at 253.465 kb on - strand, within GFF3501 at 253.558 kb on - strand at 253.558 kb on - strand at 253.566 kb on + strand at 253.567 kb on - strand at 253.879 kb on - strand at 254.023 kb on + strand, within GFF3502
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 252,085 + +0.8 252,168 - -1.1 252,168 - -0.3 252,168 - -0.2 252,173 + +0.0 253,190 - -0.3 253,265 + GFF3501 0.14 +0.3 253,266 - GFF3501 0.14 -0.3 253,266 - GFF3501 0.14 -1.5 253,287 + GFF3501 0.20 +0.6 253,288 - GFF3501 0.20 -3.1 253,428 + GFF3501 0.61 +0.6 253,465 - GFF3501 0.72 -1.0 253,558 - -0.4 253,558 - +1.2 253,566 + +1.1 253,567 - +0.8 253,879 - +0.3 254,023 + GFF3502 0.51 +0.3
Or see this region's nucleotide sequence