Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3473 and GFF3474 are separated by 426 nucleotides GFF3474 and GFF3475 are separated by 391 nucleotides
GFF3473 - Serine protein kinase (prkA protein), P-loop containing, at 223,881 to 225,815
GFF3473
GFF3474 - MltA-interacting protein MipA, at 226,242 to 226,988
GFF3474
GFF3475 - Transcriptional regulator, at 227,380 to 228,474
GFF3475
Position (kb) 226
227 Strain fitness (log2 ratio) -2
-1
0
1
2 at 225.290 kb on + strand, within GFF3473 at 225.370 kb on - strand, within GFF3473 at 225.544 kb on + strand, within GFF3473 at 225.545 kb on - strand, within GFF3473 at 225.545 kb on - strand, within GFF3473 at 225.665 kb on + strand at 225.688 kb on - strand at 225.809 kb on + strand at 225.809 kb on + strand at 225.862 kb on - strand at 225.868 kb on - strand at 225.889 kb on + strand at 225.987 kb on + strand at 225.993 kb on + strand at 226.019 kb on + strand at 226.153 kb on - strand at 226.358 kb on + strand, within GFF3474 at 226.402 kb on - strand, within GFF3474 at 226.446 kb on + strand, within GFF3474 at 226.447 kb on - strand, within GFF3474 at 226.447 kb on - strand, within GFF3474 at 226.634 kb on + strand, within GFF3474 at 226.655 kb on + strand, within GFF3474 at 226.758 kb on + strand, within GFF3474 at 226.768 kb on + strand, within GFF3474 at 226.850 kb on + strand, within GFF3474 at 226.879 kb on + strand, within GFF3474 at 226.879 kb on + strand, within GFF3474 at 226.880 kb on - strand, within GFF3474 at 226.881 kb on + strand, within GFF3474 at 226.886 kb on + strand, within GFF3474 at 227.075 kb on - strand at 227.108 kb on - strand at 227.285 kb on + strand at 227.288 kb on + strand at 227.625 kb on + strand, within GFF3475 at 227.626 kb on - strand, within GFF3475 at 227.659 kb on - strand, within GFF3475 at 227.827 kb on - strand, within GFF3475 at 227.958 kb on + strand, within GFF3475
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 225,290 + GFF3473 0.73 +0.8 225,370 - GFF3473 0.77 -2.0 225,544 + GFF3473 0.86 -0.5 225,545 - GFF3473 0.86 +0.5 225,545 - GFF3473 0.86 -0.8 225,665 + -1.4 225,688 - -0.7 225,809 + -1.0 225,809 + +1.0 225,862 - -0.5 225,868 - -1.1 225,889 + -0.2 225,987 + -1.4 225,993 + +1.3 226,019 + -0.5 226,153 - +0.1 226,358 + GFF3474 0.16 +0.9 226,402 - GFF3474 0.21 -1.3 226,446 + GFF3474 0.27 -0.4 226,447 - GFF3474 0.27 -0.2 226,447 - GFF3474 0.27 +1.8 226,634 + GFF3474 0.52 -1.2 226,655 + GFF3474 0.55 +0.4 226,758 + GFF3474 0.69 +1.0 226,768 + GFF3474 0.70 -0.4 226,850 + GFF3474 0.81 -0.8 226,879 + GFF3474 0.85 -1.9 226,879 + GFF3474 0.85 -2.5 226,880 - GFF3474 0.85 +1.4 226,881 + GFF3474 0.86 -1.6 226,886 + GFF3474 0.86 -0.7 227,075 - +1.2 227,108 - +1.5 227,285 + -1.5 227,288 + -0.2 227,625 + GFF3475 0.22 -0.1 227,626 - GFF3475 0.22 +0.3 227,659 - GFF3475 0.25 +1.4 227,827 - GFF3475 0.41 -1.3 227,958 + GFF3475 0.53 -0.8
Or see this region's nucleotide sequence