Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3472

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3471 and GFF3472 are separated by 640 nucleotidesGFF3472 and GFF3473 are separated by 122 nucleotides GFF3471 - Putative two-component response regulator and GGDEF family protein YeaJ, at 220,338 to 221,831 GFF3471 GFF3472 - UPF0229 protein YeaH, at 222,472 to 223,758 GFF3472 GFF3473 - Serine protein kinase (prkA protein), P-loop containing, at 223,881 to 225,815 GFF3473 Position (kb) 222 223 224Strain fitness (log2 ratio) -3 -2 -1 0 1at 221.480 kb on + strand, within GFF3471at 221.514 kb on + strand, within GFF3471at 221.719 kb on + strandat 221.720 kb on - strandat 222.264 kb on + strandat 222.334 kb on + strandat 222.416 kb on - strandat 222.474 kb on - strandat 222.602 kb on - strand, within GFF3472at 222.642 kb on - strand, within GFF3472at 222.644 kb on + strand, within GFF3472at 222.711 kb on + strand, within GFF3472at 222.737 kb on + strand, within GFF3472at 222.738 kb on - strand, within GFF3472at 222.885 kb on - strand, within GFF3472at 222.905 kb on + strand, within GFF3472at 223.311 kb on + strand, within GFF3472at 223.350 kb on + strand, within GFF3472at 223.581 kb on + strand, within GFF3472at 223.585 kb on - strand, within GFF3472at 223.680 kb on + strandat 223.681 kb on - strandat 223.759 kb on - strandat 223.838 kb on - strandat 223.942 kb on + strandat 224.012 kb on + strandat 224.181 kb on - strand, within GFF3473at 224.291 kb on - strand, within GFF3473at 224.417 kb on - strand, within GFF3473at 224.540 kb on + strand, within GFF3473at 224.540 kb on + strand, within GFF3473at 224.590 kb on + strand, within GFF3473at 224.622 kb on - strand, within GFF3473

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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221,480 + GFF3471 0.76 +0.3
221,514 + GFF3471 0.79 -0.1
221,719 + +1.2
221,720 - -0.1
222,264 + +0.7
222,334 + -0.2
222,416 - -1.1
222,474 - -0.7
222,602 - GFF3472 0.10 -0.1
222,642 - GFF3472 0.13 +0.0
222,644 + GFF3472 0.13 +0.1
222,711 + GFF3472 0.19 +0.0
222,737 + GFF3472 0.21 +0.4
222,738 - GFF3472 0.21 +1.7
222,885 - GFF3472 0.32 -0.5
222,905 + GFF3472 0.34 -1.1
223,311 + GFF3472 0.65 +0.3
223,350 + GFF3472 0.68 -0.1
223,581 + GFF3472 0.86 +0.3
223,585 - GFF3472 0.86 +0.4
223,680 + -1.5
223,681 - -3.6
223,759 - +1.3
223,838 - +0.1
223,942 + +0.0
224,012 + +0.4
224,181 - GFF3473 0.16 +0.4
224,291 - GFF3473 0.21 -0.7
224,417 - GFF3473 0.28 -0.5
224,540 + GFF3473 0.34 +0.5
224,540 + GFF3473 0.34 +0.3
224,590 + GFF3473 0.37 -1.0
224,622 - GFF3473 0.38 -0.9

Or see this region's nucleotide sequence