Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3416 and GFF3417 are separated by 3 nucleotides GFF3417 and GFF3418 are separated by 86 nucleotides GFF3418 and GFF3419 are separated by 53 nucleotides GFF3419 and GFF3420 are separated by 11 nucleotides GFF3420 and GFF3421 overlap by 4 nucleotides
GFF3416 - Adenylosuccinate lyase (EC 4.3.2.2), at 181,539 to 182,909
GFF3416
GFF3417 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 182,913 to 183,554
GFF3417
GFF3418 - tRNA-specific 2-thiouridylase MnmA, at 183,641 to 184,747
GFF3418
GFF3419 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 184,801 to 185,262
GFF3419
GFF3420 - FIG01056067: hypothetical protein, at 185,274 to 185,603
GFF3420
GFF3421 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 185,600 to 186,337
GFF3421
Position (kb) 183
184
185 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 182.982 kb on - strand, within GFF3417 at 183.143 kb on - strand, within GFF3417 at 183.166 kb on - strand, within GFF3417 at 183.234 kb on - strand, within GFF3417 at 183.291 kb on - strand, within GFF3417 at 183.291 kb on - strand, within GFF3417 at 183.405 kb on - strand, within GFF3417 at 183.572 kb on - strand at 184.845 kb on + strand at 184.874 kb on + strand, within GFF3419 at 185.215 kb on - strand, within GFF3419 at 185.227 kb on + strand at 185.227 kb on + strand at 185.262 kb on + strand at 185.285 kb on + strand at 185.677 kb on - strand, within GFF3421
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 182,982 - GFF3417 0.11 +3.1 183,143 - GFF3417 0.36 +2.3 183,166 - GFF3417 0.39 +1.6 183,234 - GFF3417 0.50 +3.2 183,291 - GFF3417 0.59 +3.6 183,291 - GFF3417 0.59 +3.7 183,405 - GFF3417 0.77 +3.5 183,572 - -0.6 184,845 + +0.5 184,874 + GFF3419 0.16 -0.2 185,215 - GFF3419 0.90 -1.7 185,227 + +1.1 185,227 + -3.4 185,262 + +1.8 185,285 + -0.3 185,677 - GFF3421 0.10 -1.0
Or see this region's nucleotide sequence