Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3390 and GFF3391 are separated by 24 nucleotides GFF3391 and GFF3392 are separated by 227 nucleotides GFF3392 and GFF3393 are separated by 178 nucleotides GFF3393 and GFF3394 are separated by 74 nucleotides
GFF3390 - Beta N-acetyl-glucosaminidase (EC 3.2.1.52), at 154,659 to 155,684
GFF3390
GFF3391 - YcfP protein: probably an esterase that is part of a salvage cluster, at 155,709 to 156,251
GFF3391
GFF3392 - NADH dehydrogenase (EC 1.6.99.3), at 156,479 to 157,810
GFF3392
GFF3393 - FIG01200701: possible membrane protein, at 157,989 to 158,528
GFF3393
GFF3394 - putative transcriptional repressor, at 158,603 to 159,238
GFF3394
Position (kb) 156
157
158 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 155.480 kb on + strand, within GFF3390 at 155.573 kb on + strand, within GFF3390 at 155.715 kb on - strand at 155.741 kb on + strand at 155.808 kb on + strand, within GFF3391 at 155.897 kb on - strand, within GFF3391 at 156.030 kb on + strand, within GFF3391 at 156.225 kb on - strand at 156.708 kb on - strand, within GFF3392 at 156.919 kb on + strand, within GFF3392 at 156.920 kb on - strand, within GFF3392 at 157.076 kb on - strand, within GFF3392 at 157.097 kb on - strand, within GFF3392 at 157.097 kb on - strand, within GFF3392 at 157.100 kb on + strand, within GFF3392 at 157.100 kb on + strand, within GFF3392 at 157.106 kb on + strand, within GFF3392 at 157.107 kb on - strand, within GFF3392 at 157.162 kb on + strand, within GFF3392 at 157.186 kb on + strand, within GFF3392 at 157.433 kb on - strand, within GFF3392 at 157.436 kb on + strand, within GFF3392 at 157.694 kb on + strand at 157.695 kb on - strand at 157.706 kb on + strand at 157.707 kb on - strand at 157.711 kb on + strand at 157.742 kb on - strand at 157.943 kb on + strand at 158.352 kb on + strand, within GFF3393 at 158.352 kb on + strand, within GFF3393 at 158.352 kb on + strand, within GFF3393 at 158.352 kb on + strand, within GFF3393 at 158.352 kb on + strand, within GFF3393 at 158.352 kb on + strand, within GFF3393 at 158.376 kb on - strand, within GFF3393 at 158.386 kb on - strand, within GFF3393 at 158.462 kb on + strand, within GFF3393 at 158.717 kb on + strand, within GFF3394 at 158.720 kb on - strand, within GFF3394 at 158.721 kb on + strand, within GFF3394
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 155,480 + GFF3390 0.80 +0.3 155,573 + GFF3390 0.89 +0.2 155,715 - +0.0 155,741 + -2.8 155,808 + GFF3391 0.18 +0.5 155,897 - GFF3391 0.35 -1.0 156,030 + GFF3391 0.59 +2.3 156,225 - -2.1 156,708 - GFF3392 0.17 -1.4 156,919 + GFF3392 0.33 +0.2 156,920 - GFF3392 0.33 -1.3 157,076 - GFF3392 0.45 -1.3 157,097 - GFF3392 0.46 +0.0 157,097 - GFF3392 0.46 +0.7 157,100 + GFF3392 0.47 +0.1 157,100 + GFF3392 0.47 -0.5 157,106 + GFF3392 0.47 -1.0 157,107 - GFF3392 0.47 +1.9 157,162 + GFF3392 0.51 -1.4 157,186 + GFF3392 0.53 -2.4 157,433 - GFF3392 0.72 -0.6 157,436 + GFF3392 0.72 -2.2 157,694 + -1.8 157,695 - -1.5 157,706 + -2.7 157,707 - -2.0 157,711 + -3.2 157,742 - -0.4 157,943 + +0.5 158,352 + GFF3393 0.67 +1.0 158,352 + GFF3393 0.67 +0.7 158,352 + GFF3393 0.67 +1.4 158,352 + GFF3393 0.67 +1.5 158,352 + GFF3393 0.67 -0.8 158,352 + GFF3393 0.67 +0.8 158,376 - GFF3393 0.72 +0.2 158,386 - GFF3393 0.74 +1.1 158,462 + GFF3393 0.88 +1.7 158,717 + GFF3394 0.18 +1.3 158,720 - GFF3394 0.18 -0.4 158,721 + GFF3394 0.19 +0.6
Or see this region's nucleotide sequence