Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3379 and GFF3380 overlap by 11 nucleotides GFF3380 and GFF3381 overlap by 4 nucleotides GFF3381 and GFF3382 are separated by 10 nucleotides GFF3382 and GFF3383 are separated by 23 nucleotides
GFF3379 - FIG004453: protein YceG like, at 144,664 to 145,686
GFF3379
GFF3380 - Thymidylate kinase (EC 2.7.4.9), at 145,676 to 146,317
GFF3380
GFF3381 - DNA polymerase III delta prime subunit (EC 2.7.7.7), at 146,314 to 147,318
GFF3381
GFF3382 - Putative deoxyribonuclease YcfH, at 147,329 to 148,126
GFF3382
GFF3383 - hypothetical protein, at 148,150 to 148,287
GFF3383
Position (kb) 146
147
148 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 145.360 kb on - strand, within GFF3379 at 145.429 kb on + strand, within GFF3379 at 145.430 kb on - strand, within GFF3379 at 145.430 kb on - strand, within GFF3379 at 145.430 kb on - strand, within GFF3379 at 145.431 kb on + strand, within GFF3379 at 145.484 kb on - strand, within GFF3379 at 145.485 kb on + strand, within GFF3379 at 147.372 kb on - strand at 147.386 kb on - strand at 147.551 kb on + strand, within GFF3382 at 147.624 kb on - strand, within GFF3382 at 147.668 kb on - strand, within GFF3382 at 147.739 kb on + strand, within GFF3382 at 147.786 kb on + strand, within GFF3382 at 147.790 kb on - strand, within GFF3382 at 147.845 kb on + strand, within GFF3382 at 147.927 kb on + strand, within GFF3382 at 147.928 kb on - strand, within GFF3382 at 147.930 kb on + strand, within GFF3382 at 147.950 kb on + strand, within GFF3382 at 148.016 kb on + strand, within GFF3382 at 148.093 kb on + strand at 148.307 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 145,360 - GFF3379 0.68 -1.7 145,429 + GFF3379 0.75 +0.8 145,430 - GFF3379 0.75 -1.3 145,430 - GFF3379 0.75 -1.8 145,430 - GFF3379 0.75 -0.3 145,431 + GFF3379 0.75 -1.8 145,484 - GFF3379 0.80 -1.3 145,485 + GFF3379 0.80 -0.5 147,372 - +0.4 147,386 - -0.4 147,551 + GFF3382 0.28 -0.3 147,624 - GFF3382 0.37 -2.9 147,668 - GFF3382 0.42 +0.2 147,739 + GFF3382 0.51 +0.9 147,786 + GFF3382 0.57 -0.7 147,790 - GFF3382 0.58 -2.2 147,845 + GFF3382 0.65 -0.7 147,927 + GFF3382 0.75 +0.4 147,928 - GFF3382 0.75 +1.0 147,930 + GFF3382 0.75 -2.0 147,950 + GFF3382 0.78 -0.2 148,016 + GFF3382 0.86 -0.8 148,093 + -0.8 148,307 - -1.6
Or see this region's nucleotide sequence