Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3373

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3372 and GFF3373 are separated by 79 nucleotidesGFF3373 and GFF3374 are separated by 15 nucleotidesGFF3374 and GFF3375 are separated by 12 nucleotides GFF3372 - Phosphate:acyl-ACP acyltransferase PlsX, at 138,235 to 139,284 GFF3372 GFF3373 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41), at 139,364 to 140,317 GFF3373 GFF3374 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 140,333 to 141,262 GFF3374 GFF3375 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 141,275 to 142,009 GFF3375 Position (kb) 139 140 141Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 138.409 kb on - strand, within GFF3372at 138.669 kb on + strand, within GFF3372at 139.069 kb on + strand, within GFF3372at 139.069 kb on + strand, within GFF3372at 139.070 kb on - strand, within GFF3372at 139.071 kb on + strand, within GFF3372at 139.220 kb on + strandat 139.282 kb on + strandat 139.285 kb on + strandat 139.324 kb on + strandat 139.336 kb on + strandat 139.338 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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138,409 - GFF3372 0.17 +6.4
138,669 + GFF3372 0.41 +1.6
139,069 + GFF3372 0.79 -1.3
139,069 + GFF3372 0.79 +3.2
139,070 - GFF3372 0.80 +2.3
139,071 + GFF3372 0.80 +4.6
139,220 + +3.7
139,282 + +4.7
139,285 + +5.3
139,324 + +2.9
139,336 + -0.2
139,338 + +3.2

Or see this region's nucleotide sequence