Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3361 and GFF3362 overlap by 1 nucleotides GFF3362 and GFF3363 are separated by 64 nucleotides
GFF3361 - Flagellar P-ring protein FlgI, at 125,408 to 126,505
GFF3361
GFF3362 - Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-), at 126,505 to 127,455
GFF3362
GFF3363 - Flagellar hook-associated protein FlgK, at 127,520 to 129,181
GFF3363
Position (kb) 126
127
128 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 125.641 kb on + strand, within GFF3361 at 125.642 kb on - strand, within GFF3361 at 125.773 kb on - strand, within GFF3361 at 125.784 kb on + strand, within GFF3361 at 125.807 kb on + strand, within GFF3361 at 125.808 kb on - strand, within GFF3361 at 125.808 kb on - strand, within GFF3361 at 125.827 kb on + strand, within GFF3361 at 125.888 kb on - strand, within GFF3361 at 125.962 kb on + strand, within GFF3361 at 126.076 kb on - strand, within GFF3361 at 126.130 kb on + strand, within GFF3361 at 126.220 kb on + strand, within GFF3361 at 126.481 kb on - strand at 126.520 kb on - strand at 127.220 kb on - strand, within GFF3362 at 127.241 kb on + strand, within GFF3362 at 127.280 kb on - strand, within GFF3362 at 127.454 kb on - strand at 127.555 kb on + strand at 127.579 kb on + strand at 127.579 kb on + strand at 127.580 kb on - strand at 127.621 kb on + strand at 127.636 kb on + strand at 127.684 kb on + strand at 127.707 kb on + strand, within GFF3363 at 127.718 kb on + strand, within GFF3363 at 127.718 kb on + strand, within GFF3363 at 127.718 kb on + strand, within GFF3363 at 127.743 kb on + strand, within GFF3363 at 127.743 kb on + strand, within GFF3363 at 127.941 kb on + strand, within GFF3363 at 128.175 kb on + strand, within GFF3363 at 128.193 kb on - strand, within GFF3363 at 128.193 kb on - strand, within GFF3363 at 128.218 kb on + strand, within GFF3363 at 128.218 kb on + strand, within GFF3363 at 128.298 kb on - strand, within GFF3363 at 128.298 kb on - strand, within GFF3363 at 128.404 kb on + strand, within GFF3363 at 128.405 kb on - strand, within GFF3363 at 128.408 kb on - strand, within GFF3363
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 125,641 + GFF3361 0.21 -0.5 125,642 - GFF3361 0.21 +1.5 125,773 - GFF3361 0.33 +2.6 125,784 + GFF3361 0.34 +1.7 125,807 + GFF3361 0.36 +1.3 125,808 - GFF3361 0.36 -0.6 125,808 - GFF3361 0.36 +1.5 125,827 + GFF3361 0.38 -1.6 125,888 - GFF3361 0.44 +1.4 125,962 + GFF3361 0.50 +1.1 126,076 - GFF3361 0.61 +1.9 126,130 + GFF3361 0.66 -1.1 126,220 + GFF3361 0.74 +3.3 126,481 - +2.9 126,520 - +1.7 127,220 - GFF3362 0.75 +1.2 127,241 + GFF3362 0.77 +1.0 127,280 - GFF3362 0.81 +1.4 127,454 - -2.5 127,555 + +1.5 127,579 + -0.7 127,579 + +1.6 127,580 - +1.5 127,621 + +2.2 127,636 + +0.6 127,684 + +1.0 127,707 + GFF3363 0.11 +1.3 127,718 + GFF3363 0.12 +0.7 127,718 + GFF3363 0.12 -0.9 127,718 + GFF3363 0.12 +0.9 127,743 + GFF3363 0.13 +0.7 127,743 + GFF3363 0.13 +0.3 127,941 + GFF3363 0.25 +1.4 128,175 + GFF3363 0.39 +0.1 128,193 - GFF3363 0.40 +2.9 128,193 - GFF3363 0.40 +1.4 128,218 + GFF3363 0.42 +2.1 128,218 + GFF3363 0.42 +1.7 128,298 - GFF3363 0.47 -0.9 128,298 - GFF3363 0.47 -1.9 128,404 + GFF3363 0.53 +1.7 128,405 - GFF3363 0.53 +2.0 128,408 - GFF3363 0.53 -2.1
Or see this region's nucleotide sequence