Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3218

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3216 and GFF3217 are separated by 34 nucleotidesGFF3217 and GFF3218 overlap by 4 nucleotides GFF3216 - Pantothenate kinase (EC 2.7.1.33), at 1,029 to 1,979 GFF3216 GFF3217 - Biotin operon repressor / Biotin-protein ligase (EC 6.3.4.15), at 2,014 to 2,976 GFF3217 GFF3218 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 2,973 to 4,001 GFF3218 Position (kb) 2 3 4 5Strain fitness (log2 ratio) -1 0 1at 4.067 kb on + strandat 4.067 kb on + strandat 4.068 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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4,067 + -1.0
4,067 + -1.2
4,068 - -1.5

Or see this region's nucleotide sequence