Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3216

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3219 and GFF3220 are separated by 6 nucleotidesGFF3220 and GFF3221 are separated by 116 nucleotidesGFF3221 and GFF3222 are separated by 8 nucleotidesGFF3222 and GFF3216 are separated by 401 nucleotidesGFF3216 and GFF3217 are separated by 34 nucleotidesGFF3217 and GFF3218 overlap by 4 nucleotides GFF3219 - tRNA-Thr-GGT, at 186 to 261 GFF3219 GFF3220 - tRNA-Gly-TCC, at 268 to 342 GFF3220 GFF3221 - tRNA-Tyr-GTA, at 459 to 543 GFF3221 GFF3222 - tRNA-Thr-TGT, at 552 to 627 GFF3222 GFF3216 - Pantothenate kinase (EC 2.7.1.33), at 1,029 to 1,979 GFF3216 GFF3217 - Biotin operon repressor / Biotin-protein ligase (EC 6.3.4.15), at 2,014 to 2,976 GFF3217 GFF3218 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 2,973 to 4,001 GFF3218 Position (kb) 1 2Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 0.196 kb on - strand, within GFF3219at 0.254 kb on - strandat 0.254 kb on - strandat 0.508 kb on - strand, within GFF3221at 0.843 kb on - strandat 0.845 kb on - strandat 0.897 kb on + strandat 0.917 kb on + strandat 1.028 kb on + strandat 1.028 kb on + strandat 1.029 kb on - strandat 1.029 kb on - strandat 1.036 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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196 - GFF3219 0.13 +3.0
254 - +3.0
254 - +3.2
508 - GFF3221 0.58 +0.1
843 - +0.3
845 - -0.1
897 + -0.9
917 + +0.7
1,028 + -2.3
1,028 + -3.2
1,029 - +2.5
1,029 - +0.6
1,036 + -2.5

Or see this region's nucleotide sequence