Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3158 and GFF3159 are separated by 14 nucleotides GFF3159 and GFF3160 are separated by 170 nucleotides GFF3160 and GFF3161 are separated by 436 nucleotides GFF3161 and GFF3162 are separated by 73 nucleotides
GFF3158 - Periplasmic thiol:disulfide interchange protein, DsbA-like, at 161,452 to 162,123
GFF3158
GFF3159 - Inner membrane thiol:disulfide oxidoreductase, DsbB-like, at 162,138 to 162,815
GFF3159
GFF3160 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12), at 162,986 to 163,639
GFF3160
GFF3161 - hypothetical protein, at 164,076 to 164,375
GFF3161
GFF3162 - glycogen synthesis protein GlgS, at 164,449 to 164,658
GFF3162
Position (kb)
162
163
164 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 162.047 kb on - strand, within GFF3158 at 162.047 kb on - strand, within GFF3158 at 162.051 kb on + strand, within GFF3158 at 162.052 kb on - strand, within GFF3158 at 162.052 kb on - strand, within GFF3158 at 162.056 kb on - strand at 162.285 kb on + strand, within GFF3159 at 162.318 kb on + strand, within GFF3159 at 162.347 kb on - strand, within GFF3159 at 162.370 kb on + strand, within GFF3159 at 162.441 kb on - strand, within GFF3159 at 162.449 kb on + strand, within GFF3159 at 162.458 kb on + strand, within GFF3159 at 162.478 kb on + strand, within GFF3159 at 162.482 kb on + strand, within GFF3159 at 162.483 kb on - strand, within GFF3159 at 162.550 kb on + strand, within GFF3159 at 162.647 kb on - strand, within GFF3159 at 162.690 kb on + strand, within GFF3159 at 162.734 kb on + strand, within GFF3159 at 162.734 kb on + strand, within GFF3159 at 162.734 kb on + strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.735 kb on - strand, within GFF3159 at 162.826 kb on + strand at 162.917 kb on + strand at 162.917 kb on + strand at 163.953 kb on - strand at 163.955 kb on - strand at 163.985 kb on + strand at 163.985 kb on + strand at 164.037 kb on - strand at 164.048 kb on + strand at 164.049 kb on - strand at 164.405 kb on + strand at 164.405 kb on + strand at 164.406 kb on - strand at 164.450 kb on + strand at 164.541 kb on + strand, within GFF3162 at 164.623 kb on - strand, within GFF3162
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 162,047 - GFF3158 0.89 -1.6 162,047 - GFF3158 0.89 -2.1 162,051 + GFF3158 0.89 +0.7 162,052 - GFF3158 0.89 -1.2 162,052 - GFF3158 0.89 +1.6 162,056 - +0.1 162,285 + GFF3159 0.22 -0.1 162,318 + GFF3159 0.27 -0.0 162,347 - GFF3159 0.31 +0.0 162,370 + GFF3159 0.34 +0.9 162,441 - GFF3159 0.45 -0.8 162,449 + GFF3159 0.46 +0.7 162,458 + GFF3159 0.47 +0.7 162,478 + GFF3159 0.50 -0.3 162,482 + GFF3159 0.51 -0.3 162,483 - GFF3159 0.51 +2.1 162,550 + GFF3159 0.61 -1.5 162,647 - GFF3159 0.75 -1.2 162,690 + GFF3159 0.81 +1.8 162,734 + GFF3159 0.88 +0.3 162,734 + GFF3159 0.88 +1.1 162,734 + GFF3159 0.88 +0.2 162,735 - GFF3159 0.88 -2.3 162,735 - GFF3159 0.88 +0.8 162,735 - GFF3159 0.88 +0.4 162,735 - GFF3159 0.88 -0.6 162,735 - GFF3159 0.88 +0.6 162,826 + +0.5 162,917 + -2.4 162,917 + +1.4 163,953 - +2.5 163,955 - +2.2 163,985 + +0.5 163,985 + -0.1 164,037 - +1.8 164,048 + +0.1 164,049 - +1.0 164,405 + -3.3 164,405 + +1.2 164,406 - -0.8 164,450 + +0.4 164,541 + GFF3162 0.44 +1.9 164,623 - GFF3162 0.83 +0.1
Or see this region's nucleotide sequence