Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3127 and GFF3128 are separated by 157 nucleotides GFF3128 and GFF3129 are separated by 202 nucleotides GFF3129 and GFF3130 are separated by 487 nucleotides
GFF3127 - FIG01046325: hypothetical protein, at 133,063 to 133,518
GFF3127
GFF3128 - hypothetical protein, at 133,676 to 133,870
GFF3128
GFF3129 - Probable Fe-S oxidoreductase family 2, at 134,073 to 136,244
GFF3129
GFF3130 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 136,732 to 137,715
GFF3130
Position (kb) 134
135
136
137 Strain fitness (log2 ratio) -1
0
1
2 at 133.207 kb on + strand, within GFF3127 at 133.334 kb on + strand, within GFF3127 at 133.548 kb on + strand at 133.617 kb on - strand at 134.411 kb on - strand, within GFF3129 at 134.413 kb on + strand, within GFF3129 at 134.447 kb on - strand, within GFF3129 at 134.548 kb on + strand, within GFF3129 at 134.843 kb on - strand, within GFF3129 at 134.913 kb on - strand, within GFF3129 at 134.924 kb on + strand, within GFF3129 at 134.925 kb on - strand, within GFF3129 at 134.926 kb on + strand, within GFF3129 at 134.926 kb on + strand, within GFF3129 at 134.926 kb on + strand, within GFF3129 at 134.926 kb on + strand, within GFF3129 at 134.927 kb on - strand, within GFF3129 at 134.927 kb on - strand, within GFF3129 at 135.014 kb on + strand, within GFF3129 at 135.015 kb on - strand, within GFF3129 at 135.101 kb on + strand, within GFF3129 at 135.101 kb on + strand, within GFF3129 at 135.102 kb on - strand, within GFF3129 at 135.325 kb on + strand, within GFF3129 at 135.326 kb on - strand, within GFF3129 at 135.326 kb on - strand, within GFF3129 at 135.326 kb on - strand, within GFF3129 at 135.373 kb on + strand, within GFF3129 at 135.374 kb on - strand, within GFF3129 at 135.507 kb on - strand, within GFF3129 at 135.972 kb on - strand, within GFF3129 at 136.041 kb on - strand at 136.081 kb on + strand at 136.236 kb on - strand at 136.291 kb on + strand at 136.293 kb on + strand at 136.293 kb on + strand at 136.335 kb on + strand at 136.336 kb on - strand at 136.718 kb on - strand at 136.873 kb on + strand, within GFF3130 at 136.873 kb on + strand, within GFF3130 at 136.874 kb on - strand, within GFF3130 at 136.917 kb on + strand, within GFF3130 at 137.039 kb on - strand, within GFF3130 at 137.041 kb on - strand, within GFF3130 at 137.087 kb on - strand, within GFF3130 at 137.093 kb on + strand, within GFF3130
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 133,207 + GFF3127 0.32 -0.5 133,334 + GFF3127 0.59 -0.2 133,548 + +1.3 133,617 - +1.3 134,411 - GFF3129 0.16 +0.5 134,413 + GFF3129 0.16 +1.1 134,447 - GFF3129 0.17 -0.3 134,548 + GFF3129 0.22 -1.5 134,843 - GFF3129 0.35 -0.2 134,913 - GFF3129 0.39 -0.2 134,924 + GFF3129 0.39 +1.3 134,925 - GFF3129 0.39 +0.3 134,926 + GFF3129 0.39 +1.3 134,926 + GFF3129 0.39 +0.4 134,926 + GFF3129 0.39 +1.5 134,926 + GFF3129 0.39 -0.3 134,927 - GFF3129 0.39 -1.0 134,927 - GFF3129 0.39 +1.0 135,014 + GFF3129 0.43 -0.5 135,015 - GFF3129 0.43 +2.1 135,101 + GFF3129 0.47 +2.7 135,101 + GFF3129 0.47 -0.5 135,102 - GFF3129 0.47 +1.8 135,325 + GFF3129 0.58 +0.8 135,326 - GFF3129 0.58 -0.6 135,326 - GFF3129 0.58 +0.6 135,326 - GFF3129 0.58 -0.3 135,373 + GFF3129 0.60 +1.2 135,374 - GFF3129 0.60 +0.1 135,507 - GFF3129 0.66 +1.1 135,972 - GFF3129 0.87 -0.1 136,041 - +0.7 136,081 + +0.7 136,236 - -1.1 136,291 + -1.3 136,293 + +0.2 136,293 + +0.6 136,335 + +0.5 136,336 - +1.5 136,718 - -1.2 136,873 + GFF3130 0.14 -0.5 136,873 + GFF3130 0.14 +0.0 136,874 - GFF3130 0.14 +0.2 136,917 + GFF3130 0.19 -0.4 137,039 - GFF3130 0.31 -0.2 137,041 - GFF3130 0.31 +0.8 137,087 - GFF3130 0.36 +0.8 137,093 + GFF3130 0.37 -0.8
Or see this region's nucleotide sequence