Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF309 and GFF310 are separated by 332 nucleotides GFF310 and GFF311 are separated by 65 nucleotides GFF311 and GFF312 are separated by 75 nucleotides
GFF309 - Putative transcriptional regulator of unknown carbohydrate utilization cluster, GntR family, at 27,896 to 28,639
GFF309
GFF310 - Putative carbohydrate PTS system, IIA component (EC 2.7.1.69), at 28,972 to 29,130
GFF310
GFF311 - Putative carbohydrate PTS system, IIB component (EC 2.7.1.69), at 29,196 to 29,414
GFF311
GFF312 - Putative carbohydrate PTS system, IIC component (EC 2.7.1.69), at 29,490 to 30,848
GFF312
Position (kb) 29
30 Strain fitness (log2 ratio) -2
-1
0
1
2 at 28.216 kb on - strand, within GFF309 at 28.249 kb on - strand, within GFF309 at 28.930 kb on - strand at 28.971 kb on + strand at 29.143 kb on + strand at 29.144 kb on - strand at 29.501 kb on - strand at 29.507 kb on + strand at 29.674 kb on + strand, within GFF312 at 29.678 kb on + strand, within GFF312 at 29.679 kb on - strand, within GFF312 at 29.689 kb on + strand, within GFF312 at 29.825 kb on + strand, within GFF312 at 29.825 kb on + strand, within GFF312 at 29.849 kb on + strand, within GFF312 at 29.858 kb on + strand, within GFF312 at 29.859 kb on - strand, within GFF312 at 29.859 kb on - strand, within GFF312 at 29.883 kb on + strand, within GFF312 at 30.019 kb on - strand, within GFF312 at 30.195 kb on - strand, within GFF312 at 30.228 kb on + strand, within GFF312 at 30.342 kb on - strand, within GFF312 at 30.363 kb on + strand, within GFF312
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 28,216 - GFF309 0.43 +1.8 28,249 - GFF309 0.47 -1.8 28,930 - -0.7 28,971 + +1.4 29,143 + -1.8 29,144 - +0.4 29,501 - +0.7 29,507 + +0.5 29,674 + GFF312 0.14 +0.6 29,678 + GFF312 0.14 -0.0 29,679 - GFF312 0.14 +0.1 29,689 + GFF312 0.15 -0.4 29,825 + GFF312 0.25 +1.1 29,825 + GFF312 0.25 -0.9 29,849 + GFF312 0.26 -0.1 29,858 + GFF312 0.27 +0.1 29,859 - GFF312 0.27 +0.6 29,859 - GFF312 0.27 +0.1 29,883 + GFF312 0.29 +1.3 30,019 - GFF312 0.39 -0.1 30,195 - GFF312 0.52 +0.4 30,228 + GFF312 0.54 -0.6 30,342 - GFF312 0.63 -0.4 30,363 + GFF312 0.64 -1.3
Or see this region's nucleotide sequence