Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3039 and GFF3040 are separated by 143 nucleotides GFF3040 and GFF3041 are separated by 86 nucleotides GFF3041 and GFF3042 are separated by 333 nucleotides GFF3042 and GFF3043 are separated by 148 nucleotides
GFF3039 - Putative outer membrane lipoprotein, at 53,186 to 53,917
GFF3039
GFF3040 - Biosynthetic arginine decarboxylase (EC 4.1.1.19), at 54,061 to 55,959
GFF3040
GFF3041 - hypothetical protein, at 56,046 to 56,177
GFF3041
GFF3042 - hypothetical protein STY3242, at 56,511 to 56,678
GFF3042
GFF3043 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 56,827 to 57,981
GFF3043
Position (kb) 54
55
56 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 53.094 kb on + strand at 53.094 kb on + strand at 53.318 kb on + strand, within GFF3039 at 53.393 kb on - strand, within GFF3039 at 53.443 kb on - strand, within GFF3039 at 53.579 kb on - strand, within GFF3039 at 53.965 kb on + strand at 54.052 kb on + strand at 54.155 kb on - strand at 54.204 kb on - strand at 54.384 kb on - strand, within GFF3040 at 54.426 kb on + strand, within GFF3040 at 54.429 kb on - strand, within GFF3040 at 54.429 kb on - strand, within GFF3040 at 54.429 kb on - strand, within GFF3040 at 54.459 kb on + strand, within GFF3040 at 54.459 kb on + strand, within GFF3040 at 54.460 kb on - strand, within GFF3040 at 54.462 kb on + strand, within GFF3040 at 54.462 kb on + strand, within GFF3040 at 54.810 kb on + strand, within GFF3040 at 54.915 kb on + strand, within GFF3040 at 54.931 kb on - strand, within GFF3040 at 54.931 kb on - strand, within GFF3040 at 55.049 kb on + strand, within GFF3040 at 55.080 kb on + strand, within GFF3040 at 55.158 kb on - strand, within GFF3040 at 55.158 kb on - strand, within GFF3040 at 55.376 kb on - strand, within GFF3040 at 55.464 kb on + strand, within GFF3040 at 55.515 kb on + strand, within GFF3040 at 55.516 kb on - strand, within GFF3040 at 55.517 kb on + strand, within GFF3040 at 55.525 kb on - strand, within GFF3040 at 55.690 kb on - strand, within GFF3040 at 55.691 kb on + strand, within GFF3040 at 55.862 kb on - strand at 55.893 kb on - strand at 55.896 kb on - strand at 55.899 kb on + strand at 55.922 kb on + strand at 55.923 kb on - strand at 55.981 kb on + strand at 55.981 kb on + strand at 55.981 kb on + strand at 55.981 kb on + strand at 55.982 kb on - strand at 55.982 kb on - strand at 55.982 kb on - strand at 55.984 kb on + strand at 55.985 kb on - strand at 55.985 kb on - strand at 56.037 kb on + strand at 56.038 kb on - strand at 56.038 kb on - strand at 56.080 kb on - strand, within GFF3041 at 56.177 kb on + strand at 56.193 kb on + strand at 56.209 kb on + strand at 56.316 kb on + strand at 56.319 kb on - strand at 56.401 kb on - strand at 56.521 kb on - strand at 56.542 kb on - strand, within GFF3042 at 56.542 kb on - strand, within GFF3042 at 56.714 kb on + strand at 56.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 53,094 + -0.1 53,094 + +1.0 53,318 + GFF3039 0.18 -1.2 53,393 - GFF3039 0.28 -3.1 53,443 - GFF3039 0.35 -0.3 53,579 - GFF3039 0.54 +0.5 53,965 + -0.2 54,052 + -0.9 54,155 - +0.7 54,204 - -3.2 54,384 - GFF3040 0.17 -0.8 54,426 + GFF3040 0.19 -3.7 54,429 - GFF3040 0.19 -3.6 54,429 - GFF3040 0.19 +0.8 54,429 - GFF3040 0.19 +1.0 54,459 + GFF3040 0.21 -1.8 54,459 + GFF3040 0.21 -1.8 54,460 - GFF3040 0.21 -2.9 54,462 + GFF3040 0.21 -2.4 54,462 + GFF3040 0.21 -0.8 54,810 + GFF3040 0.39 -1.1 54,915 + GFF3040 0.45 -2.4 54,931 - GFF3040 0.46 -2.6 54,931 - GFF3040 0.46 -2.8 55,049 + GFF3040 0.52 -0.8 55,080 + GFF3040 0.54 -0.2 55,158 - GFF3040 0.58 -3.6 55,158 - GFF3040 0.58 -0.7 55,376 - GFF3040 0.69 -2.0 55,464 + GFF3040 0.74 +2.0 55,515 + GFF3040 0.77 -2.6 55,516 - GFF3040 0.77 -2.1 55,517 + GFF3040 0.77 -3.2 55,525 - GFF3040 0.77 -3.8 55,690 - GFF3040 0.86 -2.6 55,691 + GFF3040 0.86 -1.0 55,862 - -1.2 55,893 - +2.5 55,896 - -2.7 55,899 + +0.2 55,922 + -0.6 55,923 - -1.0 55,981 + -1.7 55,981 + -1.7 55,981 + +2.9 55,981 + -1.1 55,982 - -3.3 55,982 - -2.7 55,982 - -1.7 55,984 + -1.7 55,985 - -2.8 55,985 - -2.2 56,037 + -0.2 56,038 - -1.8 56,038 - -2.4 56,080 - GFF3041 0.26 -1.2 56,177 + -3.2 56,193 + -1.5 56,209 + -1.1 56,316 + -2.2 56,319 - +0.7 56,401 - -4.5 56,521 - -0.7 56,542 - GFF3042 0.18 -1.0 56,542 - GFF3042 0.18 +1.4 56,714 + +1.4 56,812 + +5.6
Or see this region's nucleotide sequence