Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3026 and GFF3027 overlap by 7 nucleotides GFF3027 and GFF3028 are separated by 43 nucleotides GFF3028 and GFF3029 are separated by 275 nucleotides
GFF3026 - ATPase component STY3232 of energizing module of queuosine-regulated ECF transporter, at 40,156 to 40,833
GFF3026
GFF3027 - ATPase component STY3233 of energizing module of queuosine-regulated ECF transporter, at 40,827 to 41,483
GFF3027
GFF3028 - Transketolase (EC 2.2.1.1), at 41,527 to 43,518
GFF3028
GFF3029 - Putative metalloprotease yggG (EC 3.4.24.-), at 43,794 to 44,552
GFF3029
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 40.610 kb on + strand, within GFF3026 at 40.611 kb on - strand, within GFF3026 at 40.709 kb on + strand, within GFF3026 at 40.889 kb on + strand at 40.890 kb on - strand at 41.140 kb on + strand, within GFF3027 at 41.195 kb on - strand, within GFF3027 at 41.348 kb on - strand, within GFF3027 at 41.447 kb on - strand at 41.447 kb on - strand at 41.447 kb on - strand at 41.655 kb on - strand at 41.862 kb on + strand, within GFF3028 at 41.894 kb on - strand, within GFF3028 at 41.934 kb on + strand, within GFF3028 at 41.935 kb on - strand, within GFF3028 at 41.998 kb on + strand, within GFF3028 at 42.006 kb on - strand, within GFF3028 at 42.144 kb on - strand, within GFF3028 at 42.308 kb on + strand, within GFF3028 at 42.308 kb on + strand, within GFF3028 at 42.312 kb on + strand, within GFF3028 at 42.974 kb on + strand, within GFF3028 at 43.071 kb on - strand, within GFF3028 at 43.090 kb on - strand, within GFF3028 at 43.162 kb on - strand, within GFF3028 at 43.206 kb on - strand, within GFF3028 at 43.241 kb on - strand, within GFF3028 at 43.241 kb on - strand, within GFF3028 at 43.241 kb on - strand, within GFF3028 at 43.305 kb on - strand, within GFF3028 at 43.305 kb on - strand, within GFF3028 at 43.488 kb on + strand at 43.506 kb on + strand at 43.609 kb on + strand at 43.609 kb on + strand at 43.678 kb on + strand at 43.679 kb on - strand at 43.679 kb on - strand at 43.721 kb on + strand at 43.722 kb on - strand at 43.811 kb on - strand at 43.865 kb on + strand at 43.865 kb on + strand at 43.866 kb on - strand at 43.907 kb on + strand, within GFF3029 at 43.908 kb on - strand, within GFF3029 at 43.999 kb on - strand, within GFF3029 at 44.064 kb on + strand, within GFF3029 at 44.064 kb on + strand, within GFF3029 at 44.065 kb on - strand, within GFF3029 at 44.065 kb on - strand, within GFF3029 at 44.067 kb on - strand, within GFF3029 at 44.118 kb on - strand, within GFF3029 at 44.212 kb on + strand, within GFF3029 at 44.377 kb on - strand, within GFF3029
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 40,610 + GFF3026 0.67 -1.2 40,611 - GFF3026 0.67 +0.9 40,709 + GFF3026 0.82 -1.1 40,889 + -1.3 40,890 - -4.1 41,140 + GFF3027 0.48 +0.2 41,195 - GFF3027 0.56 -0.2 41,348 - GFF3027 0.79 +2.4 41,447 - +0.6 41,447 - -0.3 41,447 - +0.0 41,655 - +1.0 41,862 + GFF3028 0.17 +1.2 41,894 - GFF3028 0.18 +1.9 41,934 + GFF3028 0.20 +0.8 41,935 - GFF3028 0.20 +1.9 41,998 + GFF3028 0.24 +1.8 42,006 - GFF3028 0.24 +0.2 42,144 - GFF3028 0.31 +1.6 42,308 + GFF3028 0.39 +1.4 42,308 + GFF3028 0.39 +0.6 42,312 + GFF3028 0.39 -1.2 42,974 + GFF3028 0.73 -0.2 43,071 - GFF3028 0.78 +1.7 43,090 - GFF3028 0.78 +1.8 43,162 - GFF3028 0.82 +3.0 43,206 - GFF3028 0.84 +0.7 43,241 - GFF3028 0.86 +2.2 43,241 - GFF3028 0.86 +1.4 43,241 - GFF3028 0.86 +1.3 43,305 - GFF3028 0.89 -0.2 43,305 - GFF3028 0.89 +2.4 43,488 + +0.0 43,506 + +3.8 43,609 + +1.7 43,609 + +1.6 43,678 + +0.3 43,679 - +0.5 43,679 - -1.5 43,721 + -0.2 43,722 - +0.4 43,811 - +1.8 43,865 + -1.2 43,865 + +0.4 43,866 - +1.0 43,907 + GFF3029 0.15 +0.4 43,908 - GFF3029 0.15 +0.3 43,999 - GFF3029 0.27 -1.2 44,064 + GFF3029 0.36 +0.4 44,064 + GFF3029 0.36 -0.6 44,065 - GFF3029 0.36 -0.8 44,065 - GFF3029 0.36 +0.6 44,067 - GFF3029 0.36 -0.2 44,118 - GFF3029 0.43 +0.7 44,212 + GFF3029 0.55 -0.1 44,377 - GFF3029 0.77 +1.2
Or see this region's nucleotide sequence