Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3014

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3013 and GFF3014 are separated by 265 nucleotidesGFF3014 and GFF3015 are separated by 153 nucleotides GFF3013 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 28,413 to 29,645 GFF3013 GFF3014 - Ribose 5-phosphate isomerase A (EC 5.3.1.6), at 29,911 to 30,570 GFF3014 GFF3015 - Chromosome initiation inhibitor, at 30,724 to 31,617 GFF3015 Position (kb) 29 30 31Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 28.915 kb on - strand, within GFF3013at 29.460 kb on - strand, within GFF3013at 29.537 kb on - strandat 29.709 kb on + strandat 29.709 kb on + strandat 29.709 kb on + strandat 29.709 kb on + strandat 29.710 kb on - strandat 29.897 kb on + strandat 30.571 kb on - strandat 30.609 kb on - strandat 30.643 kb on - strandat 30.666 kb on + strandat 30.667 kb on - strandat 30.682 kb on - strandat 30.749 kb on + strandat 30.770 kb on + strandat 30.855 kb on + strand, within GFF3015at 30.855 kb on + strand, within GFF3015at 30.882 kb on + strand, within GFF3015at 31.320 kb on + strand, within GFF3015

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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28,915 - GFF3013 0.41 +0.5
29,460 - GFF3013 0.85 -1.4
29,537 - +0.7
29,709 + -2.2
29,709 + -0.8
29,709 + +0.5
29,709 + -3.1
29,710 - -1.6
29,897 + -0.4
30,571 - +5.4
30,609 - +1.1
30,643 - +0.0
30,666 + -1.4
30,667 - +0.2
30,682 - +1.0
30,749 + +0.9
30,770 + +0.4
30,855 + GFF3015 0.15 +0.6
30,855 + GFF3015 0.15 -0.5
30,882 + GFF3015 0.18 -0.4
31,320 + GFF3015 0.67 -0.6

Or see this region's nucleotide sequence