Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF299

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF298 and GFF299 are separated by 3 nucleotidesGFF299 and GFF300 are separated by 1 nucleotides GFF298 - Acetolactate synthase large subunit (EC 2.2.1.6), at 15,473 to 17,161 GFF298 GFF299 - Acetolactate synthase small subunit (EC 2.2.1.6), at 17,165 to 17,455 GFF299 GFF300 - Deoxyribose operon repressor, DeoR family, at 17,457 to 18,242 GFF300 Position (kb) 17 18Strain fitness (log2 ratio) -2 -1 0 1 2 3at 16.462 kb on - strand, within GFF298at 16.644 kb on + strand, within GFF298at 16.823 kb on - strand, within GFF298at 16.903 kb on - strand, within GFF298at 16.925 kb on + strand, within GFF298at 16.928 kb on - strand, within GFF298at 17.050 kb on + strandat 17.458 kb on + strandat 17.458 kb on + strandat 17.519 kb on - strandat 17.543 kb on + strand, within GFF300at 17.724 kb on - strand, within GFF300at 17.776 kb on + strand, within GFF300at 17.832 kb on - strand, within GFF300at 18.035 kb on + strand, within GFF300at 18.056 kb on + strand, within GFF300at 18.071 kb on + strand, within GFF300at 18.268 kb on + strandat 18.323 kb on + strandat 18.324 kb on - strandat 18.325 kb on + strandat 18.325 kb on + strandat 18.326 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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16,462 - GFF298 0.59 +0.3
16,644 + GFF298 0.69 -0.8
16,823 - GFF298 0.80 +2.9
16,903 - GFF298 0.85 -0.7
16,925 + GFF298 0.86 +0.6
16,928 - GFF298 0.86 -1.0
17,050 + +0.1
17,458 + +1.4
17,458 + -1.8
17,519 - +0.2
17,543 + GFF300 0.11 -0.1
17,724 - GFF300 0.34 +0.2
17,776 + GFF300 0.41 -1.2
17,832 - GFF300 0.48 +0.3
18,035 + GFF300 0.74 -0.6
18,056 + GFF300 0.76 -1.9
18,071 + GFF300 0.78 -1.9
18,268 + +0.1
18,323 + +0.6
18,324 - -0.7
18,325 + -0.6
18,325 + +0.8
18,326 - -1.3

Or see this region's nucleotide sequence