Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2988

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2987 and GFF2988 are separated by 9 nucleotidesGFF2988 and GFF2989 are separated by 321 nucleotides GFF2987 - Lysyl-tRNA synthetase (class II) (EC 6.1.1.6), at 4,353 to 5,870 GFF2987 GFF2988 - Peptide chain release factor 2; programmed frameshift-containing, at 5,880 to 6,761 GFF2988 GFF2989 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 7,083 to 8,816 GFF2989 Position (kb) 5 6 7Strain fitness (log2 ratio) -2 -1 0 1at 7.106 kb on + strandat 7.121 kb on + strandat 7.142 kb on + strandat 7.208 kb on + strandat 7.209 kb on - strandat 7.211 kb on + strandat 7.561 kb on + strand, within GFF2989at 7.735 kb on + strand, within GFF2989at 7.735 kb on + strand, within GFF2989at 7.736 kb on - strand, within GFF2989

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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7,106 + +0.7
7,121 + +0.4
7,142 + -1.5
7,208 + +0.0
7,209 - -1.2
7,211 + -0.3
7,561 + GFF2989 0.28 +1.4
7,735 + GFF2989 0.38 -1.6
7,735 + GFF2989 0.38 +0.0
7,736 - GFF2989 0.38 -2.0

Or see this region's nucleotide sequence