Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2974 and GFF2975 are separated by 92 nucleotides GFF2975 and GFF2976 are separated by 33 nucleotides GFF2976 and GFF2977 are separated by 88 nucleotides
GFF2974 - Outer membrane stress sensor protease DegQ, serine protease, at 56,893 to 58,260
GFF2974
GFF2975 - Outer membrane stress sensor protease DegS, at 58,353 to 59,423
GFF2975
GFF2976 - Membrane protein associated with oxaloacetate decarboxylase, at 59,457 to 60,119
GFF2976
GFF2977 - Oxaloacetate decarboxylase beta chain (EC 4.1.1.3), at 60,208 to 60,438
GFF2977
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 57.628 kb on - strand, within GFF2974 at 57.706 kb on - strand, within GFF2974 at 57.835 kb on - strand, within GFF2974 at 57.861 kb on + strand, within GFF2974 at 57.940 kb on - strand, within GFF2974 at 57.940 kb on - strand, within GFF2974 at 58.248 kb on - strand at 58.270 kb on - strand at 58.314 kb on + strand at 58.314 kb on + strand at 59.569 kb on - strand, within GFF2976 at 59.751 kb on - strand, within GFF2976 at 59.751 kb on - strand, within GFF2976 at 59.751 kb on - strand, within GFF2976 at 59.753 kb on + strand, within GFF2976 at 59.754 kb on - strand, within GFF2976 at 59.754 kb on - strand, within GFF2976 at 59.848 kb on - strand, within GFF2976 at 59.870 kb on + strand, within GFF2976 at 59.870 kb on + strand, within GFF2976 at 59.870 kb on + strand, within GFF2976 at 59.873 kb on - strand, within GFF2976 at 60.007 kb on + strand, within GFF2976 at 60.007 kb on + strand, within GFF2976 at 60.008 kb on - strand, within GFF2976 at 60.008 kb on - strand, within GFF2976 at 60.008 kb on - strand, within GFF2976 at 60.008 kb on - strand, within GFF2976 at 60.010 kb on - strand, within GFF2976 at 60.091 kb on + strand at 60.091 kb on + strand at 60.091 kb on + strand at 60.092 kb on - strand at 60.092 kb on - strand at 60.092 kb on - strand at 60.126 kb on - strand at 60.156 kb on + strand at 60.209 kb on + strand at 60.210 kb on - strand at 60.225 kb on - strand at 60.225 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 57,628 - GFF2974 0.54 +0.1 57,706 - GFF2974 0.59 +0.7 57,835 - GFF2974 0.69 +1.1 57,861 + GFF2974 0.71 +0.1 57,940 - GFF2974 0.77 -0.8 57,940 - GFF2974 0.77 -1.7 58,248 - -0.3 58,270 - +0.9 58,314 + -1.9 58,314 + +0.2 59,569 - GFF2976 0.17 +0.3 59,751 - GFF2976 0.44 +0.2 59,751 - GFF2976 0.44 +0.3 59,751 - GFF2976 0.44 -0.8 59,753 + GFF2976 0.45 -0.4 59,754 - GFF2976 0.45 +1.1 59,754 - GFF2976 0.45 +3.6 59,848 - GFF2976 0.59 -0.8 59,870 + GFF2976 0.62 -3.8 59,870 + GFF2976 0.62 -3.0 59,870 + GFF2976 0.62 -1.5 59,873 - GFF2976 0.63 +0.1 60,007 + GFF2976 0.83 +0.4 60,007 + GFF2976 0.83 -0.5 60,008 - GFF2976 0.83 +0.3 60,008 - GFF2976 0.83 -0.3 60,008 - GFF2976 0.83 -0.8 60,008 - GFF2976 0.83 -0.9 60,010 - GFF2976 0.83 -1.9 60,091 + -0.1 60,091 + -1.0 60,091 + -0.4 60,092 - +0.7 60,092 - +0.2 60,092 - +0.8 60,126 - +1.0 60,156 + +0.0 60,209 + -0.3 60,210 - -2.3 60,225 - -0.3 60,225 - -1.5
Or see this region's nucleotide sequence