Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2949 and GFF2950 overlap by 4 nucleotides GFF2950 and GFF2951 are separated by 248 nucleotides GFF2951 and GFF2952 are separated by 74 nucleotides GFF2952 and GFF2953 overlap by 4 nucleotides
GFF2949 - Hypothetical ATP-binding protein UPF0042, contains P-loop, at 28,284 to 29,138
GFF2949
GFF2950 - Phosphocarrier protein, nitrogen regulation associated, at 29,135 to 29,407
GFF2950
GFF2951 - Putative uncharacterized protein YrbL, at 29,656 to 30,288
GFF2951
GFF2952 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 30,363 to 31,091
GFF2952
GFF2953 - Sigma cross-reacting protein 27A, at 31,088 to 31,741
GFF2953
Position (kb) 29
30
31 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 28.771 kb on + strand, within GFF2949 at 28.966 kb on + strand, within GFF2949 at 29.002 kb on - strand, within GFF2949 at 29.002 kb on - strand, within GFF2949 at 29.049 kb on + strand, within GFF2949 at 29.552 kb on + strand at 29.704 kb on - strand at 29.711 kb on - strand at 29.712 kb on + strand at 29.712 kb on + strand at 29.742 kb on - strand, within GFF2951 at 29.752 kb on + strand, within GFF2951 at 29.800 kb on + strand, within GFF2951 at 29.800 kb on + strand, within GFF2951 at 29.804 kb on - strand, within GFF2951 at 29.931 kb on + strand, within GFF2951 at 30.013 kb on + strand, within GFF2951 at 30.030 kb on + strand, within GFF2951 at 30.031 kb on - strand, within GFF2951 at 30.126 kb on + strand, within GFF2951 at 30.301 kb on - strand at 30.368 kb on + strand at 30.369 kb on - strand at 30.369 kb on - strand at 30.386 kb on + strand at 30.423 kb on - strand at 30.449 kb on - strand, within GFF2952 at 30.543 kb on - strand, within GFF2952 at 30.543 kb on - strand, within GFF2952 at 31.042 kb on - strand at 31.048 kb on - strand at 31.119 kb on - strand at 31.165 kb on + strand, within GFF2953 at 31.167 kb on + strand, within GFF2953 at 31.211 kb on - strand, within GFF2953 at 31.230 kb on - strand, within GFF2953 at 31.230 kb on - strand, within GFF2953 at 31.283 kb on + strand, within GFF2953
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 28,771 + GFF2949 0.57 -1.3 28,966 + GFF2949 0.80 -2.1 29,002 - GFF2949 0.84 -0.3 29,002 - GFF2949 0.84 -1.5 29,049 + GFF2949 0.89 -3.6 29,552 + +0.2 29,704 - -1.5 29,711 - -0.4 29,712 + -0.8 29,712 + +0.4 29,742 - GFF2951 0.14 +0.4 29,752 + GFF2951 0.15 -0.4 29,800 + GFF2951 0.23 -0.6 29,800 + GFF2951 0.23 -0.3 29,804 - GFF2951 0.23 +1.5 29,931 + GFF2951 0.43 +1.8 30,013 + GFF2951 0.56 +1.1 30,030 + GFF2951 0.59 +0.4 30,031 - GFF2951 0.59 +0.2 30,126 + GFF2951 0.74 -1.1 30,301 - -0.4 30,368 + +0.7 30,369 - -0.1 30,369 - -0.8 30,386 + -1.9 30,423 - +0.9 30,449 - GFF2952 0.12 -2.3 30,543 - GFF2952 0.25 -0.7 30,543 - GFF2952 0.25 +0.4 31,042 - -1.2 31,048 - -1.8 31,119 - -0.2 31,165 + GFF2953 0.12 -0.6 31,167 + GFF2953 0.12 -0.9 31,211 - GFF2953 0.19 +1.0 31,230 - GFF2953 0.22 +0.6 31,230 - GFF2953 0.22 +0.4 31,283 + GFF2953 0.30 +0.8
Or see this region's nucleotide sequence