Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2931 and GFF2932 are separated by 231 nucleotides GFF2932 and GFF2933 are separated by 57 nucleotides GFF2933 and GFF2934 are separated by 53 nucleotides GFF2934 and GFF2935 are separated by 187 nucleotides GFF2935 and GFF2936 overlap by 1 nucleotides
GFF2931 - Octaprenyl diphosphate synthase (EC 2.5.1.90), at 14,682 to 15,794
GFF2931
GFF2932 - Ner-like regulatory protein, at 16,026 to 16,313
GFF2932
GFF2933 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 16,371 to 17,630
GFF2933
GFF2934 - YrbA protein, at 17,684 to 17,938
GFF2934
GFF2935 - Uncharacterized protein YrbB, at 18,126 to 18,422
GFF2935
GFF2936 - Uncharacterized ABC transporter, auxiliary component YrbC, at 18,422 to 19,057
GFF2936
Position (kb) 16
17
18 Strain fitness (log2 ratio) -1
0
1
2 at 15.996 kb on + strand at 16.005 kb on - strand at 16.025 kb on + strand at 16.025 kb on + strand at 16.026 kb on - strand at 16.306 kb on + strand at 16.306 kb on + strand at 16.312 kb on - strand at 17.686 kb on - strand at 17.729 kb on - strand, within GFF2934 at 17.729 kb on - strand, within GFF2934 at 17.731 kb on - strand, within GFF2934 at 17.756 kb on - strand, within GFF2934 at 18.026 kb on - strand at 18.079 kb on + strand at 18.100 kb on - strand at 18.100 kb on - strand at 18.127 kb on + strand at 18.127 kb on + strand at 18.128 kb on - strand at 18.159 kb on - strand, within GFF2935 at 18.160 kb on + strand, within GFF2935 at 18.160 kb on + strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.273 kb on + strand, within GFF2935 at 18.273 kb on + strand, within GFF2935 at 18.423 kb on + strand at 18.461 kb on - strand at 18.461 kb on - strand at 18.568 kb on - strand, within GFF2936
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 15,996 + -0.6 16,005 - +0.2 16,025 + -0.1 16,025 + +1.1 16,026 - +0.1 16,306 + -0.4 16,306 + -0.9 16,312 - -0.9 17,686 - +0.3 17,729 - GFF2934 0.18 +2.2 17,729 - GFF2934 0.18 +0.4 17,731 - GFF2934 0.18 -0.7 17,756 - GFF2934 0.28 +0.3 18,026 - -0.6 18,079 + +0.5 18,100 - -0.9 18,100 - -0.7 18,127 + +0.9 18,127 + -1.0 18,128 - -0.9 18,159 - GFF2935 0.11 -0.7 18,160 + GFF2935 0.11 +0.5 18,160 + GFF2935 0.11 +0.1 18,163 - GFF2935 0.12 +0.1 18,163 - GFF2935 0.12 +0.4 18,163 - GFF2935 0.12 +1.5 18,163 - GFF2935 0.12 +1.0 18,273 + GFF2935 0.49 +0.8 18,273 + GFF2935 0.49 +1.2 18,423 + +1.0 18,461 - +0.5 18,461 - +1.1 18,568 - GFF2936 0.23 +1.4
Or see this region's nucleotide sequence