Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2850 and GFF2851 overlap by 29 nucleotides GFF2851 and GFF2852 are separated by 11 nucleotides GFF2852 and GFF2853 are separated by 167 nucleotides GFF2853 and GFF2854 are separated by 18 nucleotides GFF2854 and GFF2855 are separated by 17 nucleotides GFF2855 and GFF2856 are separated by 32 nucleotides
GFF2850 - Ribosomal RNA small subunit methyltransferase F (EC 2.1.1.-), at 373,745 to 375,286
GFF2850
GFF2851 - hypothetical protein, at 375,258 to 375,389
GFF2851
GFF2852 - FIG00638105: hypothetical protein, at 375,401 to 375,640
GFF2852
GFF2853 - FIG00638765: hypothetical protein, at 375,808 to 375,942
GFF2853
GFF2854 - Ren protein, at 375,961 to 376,611
GFF2854
GFF2855 - FIG01046004: hypothetical protein, at 376,629 to 376,802
GFF2855
GFF2856 - corresponds to STY1986 from Accession AL513382: Salmonella typhi CT18, at 376,835 to 376,999
GFF2856
Position (kb)
375
376 Strain fitness (log2 ratio)
-1
0
1
2 at 374.810 kb on + strand, within GFF2850 at 375.020 kb on - strand, within GFF2850 at 375.281 kb on - strand at 375.330 kb on + strand, within GFF2851 at 375.453 kb on + strand, within GFF2852 at 375.496 kb on + strand, within GFF2852 at 375.513 kb on + strand, within GFF2852 at 375.600 kb on - strand, within GFF2852 at 375.608 kb on - strand, within GFF2852 at 375.715 kb on - strand at 375.732 kb on - strand at 375.732 kb on - strand at 375.735 kb on + strand at 375.749 kb on + strand at 375.750 kb on - strand at 375.817 kb on + strand at 376.014 kb on + strand at 376.017 kb on - strand at 376.017 kb on - strand at 376.163 kb on + strand, within GFF2854 at 376.645 kb on + strand at 376.645 kb on + strand at 376.679 kb on + strand, within GFF2855 at 376.824 kb on - strand at 376.881 kb on - strand, within GFF2856 at 376.883 kb on - strand, within GFF2856
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 374,810 + GFF2850 0.69 -0.0 375,020 - GFF2850 0.83 -1.6 375,281 - +1.0 375,330 + GFF2851 0.55 +0.3 375,453 + GFF2852 0.22 -0.5 375,496 + GFF2852 0.40 +1.2 375,513 + GFF2852 0.47 +1.2 375,600 - GFF2852 0.83 +0.2 375,608 - GFF2852 0.86 +1.5 375,715 - -0.2 375,732 - +0.7 375,732 - +0.7 375,735 + +0.2 375,749 + +0.3 375,750 - +1.0 375,817 + -0.7 376,014 + -0.4 376,017 - -0.0 376,017 - +0.1 376,163 + GFF2854 0.31 +0.2 376,645 + +0.2 376,645 + -0.1 376,679 + GFF2855 0.29 +1.9 376,824 - -0.0 376,881 - GFF2856 0.28 +0.8 376,883 - GFF2856 0.29 -1.3
Or see this region's nucleotide sequence