Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2780

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2778 and GFF2779 overlap by 4 nucleotidesGFF2779 and GFF2780 are separated by 98 nucleotidesGFF2780 and GFF2781 are separated by 124 nucleotides GFF2778 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 301,916 to 302,764 GFF2778 GFF2779 - FIG01046434: hypothetical protein, at 302,761 to 302,931 GFF2779 GFF2780 - Ribose-phosphate pyrophosphokinase (EC 2.7.6.1), at 303,030 to 303,977 GFF2780 GFF2781 - Putative sulfate permease, at 304,102 to 305,787 GFF2781 Position (kb) 303 304Strain fitness (log2 ratio) -1 0 1 2 3at 302.811 kb on + strand, within GFF2779at 302.813 kb on + strand, within GFF2779at 302.813 kb on + strand, within GFF2779at 304.042 kb on + strandat 304.043 kb on - strandat 304.065 kb on - strandat 304.065 kb on - strandat 304.279 kb on + strand, within GFF2781at 304.290 kb on + strand, within GFF2781at 304.291 kb on - strand, within GFF2781at 304.291 kb on - strand, within GFF2781at 304.342 kb on - strand, within GFF2781at 304.357 kb on - strand, within GFF2781at 304.489 kb on + strand, within GFF2781at 304.490 kb on - strand, within GFF2781at 304.533 kb on + strand, within GFF2781at 304.536 kb on + strand, within GFF2781at 304.600 kb on + strand, within GFF2781

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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302,811 + GFF2779 0.29 +1.4
302,813 + GFF2779 0.30 +1.8
302,813 + GFF2779 0.30 +0.6
304,042 + -0.2
304,043 - -0.1
304,065 - +1.3
304,065 - +0.1
304,279 + GFF2781 0.10 -1.4
304,290 + GFF2781 0.11 -0.8
304,291 - GFF2781 0.11 -0.6
304,291 - GFF2781 0.11 -0.0
304,342 - GFF2781 0.14 +3.4
304,357 - GFF2781 0.15 +1.0
304,489 + GFF2781 0.23 -0.1
304,490 - GFF2781 0.23 -0.9
304,533 + GFF2781 0.26 +0.7
304,536 + GFF2781 0.26 +0.9
304,600 + GFF2781 0.30 -0.9

Or see this region's nucleotide sequence