Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2540

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2537 and GFF2538 are separated by 118 nucleotidesGFF2538 and GFF2539 are separated by 151 nucleotidesGFF2539 and GFF2540 overlap by 11 nucleotidesGFF2540 and GFF2541 are separated by 121 nucleotides GFF2537 - hypothetical protein, at 52,191 to 52,337 GFF2537 GFF2538 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 52,456 to 52,704 GFF2538 GFF2539 - putative phage-related secreted protein, at 52,856 to 53,071 GFF2539 GFF2540 - FIG01046001: hypothetical protein, at 53,061 to 53,393 GFF2540 GFF2541 - putative cytoplasmic protein, at 53,515 to 54,444 GFF2541 Position (kb) 53 54Strain fitness (log2 ratio) -2 -1 0 1at 52.068 kb on - strandat 52.184 kb on - strandat 52.248 kb on + strand, within GFF2537at 52.457 kb on + strandat 52.459 kb on + strandat 52.460 kb on - strandat 52.830 kb on - strandat 52.909 kb on - strand, within GFF2539at 53.194 kb on + strand, within GFF2540at 53.262 kb on + strand, within GFF2540at 53.263 kb on - strand, within GFF2540at 53.348 kb on + strand, within GFF2540at 53.349 kb on - strand, within GFF2540at 53.524 kb on + strandat 53.764 kb on - strand, within GFF2541at 54.093 kb on - strand, within GFF2541at 54.114 kb on - strand, within GFF2541at 54.281 kb on + strand, within GFF2541at 54.285 kb on + strand, within GFF2541

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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52,068 - -0.4
52,184 - +0.8
52,248 + GFF2537 0.39 -1.2
52,457 + -1.5
52,459 + -0.9
52,460 - +0.1
52,830 - -2.1
52,909 - GFF2539 0.25 -1.6
53,194 + GFF2540 0.40 +1.3
53,262 + GFF2540 0.60 +0.7
53,263 - GFF2540 0.61 +1.5
53,348 + GFF2540 0.86 +0.6
53,349 - GFF2540 0.86 +0.1
53,524 + -0.2
53,764 - GFF2541 0.27 +0.2
54,093 - GFF2541 0.62 +1.3
54,114 - GFF2541 0.64 -0.2
54,281 + GFF2541 0.82 -0.6
54,285 + GFF2541 0.83 -0.5

Or see this region's nucleotide sequence