Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2535 and GFF2536 are separated by 51 nucleotides GFF2536 and GFF2537 are separated by 179 nucleotides GFF2537 and GFF2538 are separated by 118 nucleotides GFF2538 and GFF2539 are separated by 151 nucleotides GFF2539 and GFF2540 overlap by 11 nucleotides GFF2540 and GFF2541 are separated by 121 nucleotides
GFF2535 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 50,491 to 51,570
GFF2535
GFF2536 - RidA/YER057c/UK114 superfamily, group 7, YjgH-like protein, at 51,622 to 52,011
GFF2536
GFF2537 - hypothetical protein, at 52,191 to 52,337
GFF2537
GFF2538 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 52,456 to 52,704
GFF2538
GFF2539 - putative phage-related secreted protein, at 52,856 to 53,071
GFF2539
GFF2540 - FIG01046001: hypothetical protein, at 53,061 to 53,393
GFF2540
GFF2541 - putative cytoplasmic protein, at 53,515 to 54,444
GFF2541
Position (kb)
52
53 Strain fitness (log2 ratio)
-2
-1
0
1 at 51.591 kb on - strand at 51.592 kb on + strand at 51.592 kb on + strand at 51.647 kb on - strand at 51.748 kb on - strand, within GFF2536 at 51.748 kb on - strand, within GFF2536 at 51.790 kb on + strand, within GFF2536 at 51.790 kb on + strand, within GFF2536 at 52.012 kb on - strand at 52.012 kb on - strand at 52.033 kb on - strand at 52.051 kb on + strand at 52.068 kb on - strand at 52.184 kb on - strand at 52.248 kb on + strand, within GFF2537 at 52.457 kb on + strand at 52.459 kb on + strand at 52.460 kb on - strand at 52.830 kb on - strand at 52.909 kb on - strand, within GFF2539 at 53.194 kb on + strand, within GFF2540 at 53.262 kb on + strand, within GFF2540 at 53.263 kb on - strand, within GFF2540 at 53.348 kb on + strand, within GFF2540 at 53.349 kb on - strand, within GFF2540 at 53.524 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 51,591 - +0.8 51,592 + -0.2 51,592 + +0.3 51,647 - +0.1 51,748 - GFF2536 0.32 +0.1 51,748 - GFF2536 0.32 +1.2 51,790 + GFF2536 0.43 +0.3 51,790 + GFF2536 0.43 -0.6 52,012 - -1.1 52,012 - -0.4 52,033 - +1.5 52,051 + +1.3 52,068 - -0.4 52,184 - +0.8 52,248 + GFF2537 0.39 -1.2 52,457 + -1.5 52,459 + -0.9 52,460 - +0.1 52,830 - -2.1 52,909 - GFF2539 0.25 -1.6 53,194 + GFF2540 0.40 +1.3 53,262 + GFF2540 0.60 +0.7 53,263 - GFF2540 0.61 +1.5 53,348 + GFF2540 0.86 +0.6 53,349 - GFF2540 0.86 +0.1 53,524 + -0.2
Or see this region's nucleotide sequence