Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2533 and GFF2534 are separated by 44 nucleotides GFF2534 and GFF2535 are separated by 192 nucleotides GFF2535 and GFF2536 are separated by 51 nucleotides GFF2536 and GFF2537 are separated by 179 nucleotides GFF2537 and GFF2538 are separated by 118 nucleotides
GFF2533 - Oxygenase-like protein, at 48,257 to 49,765
GFF2533
GFF2534 - Transcriptional regulator, MarR family, at 49,810 to 50,298
GFF2534
GFF2535 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-), at 50,491 to 51,570
GFF2535
GFF2536 - RidA/YER057c/UK114 superfamily, group 7, YjgH-like protein, at 51,622 to 52,011
GFF2536
GFF2537 - hypothetical protein, at 52,191 to 52,337
GFF2537
GFF2538 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 52,456 to 52,704
GFF2538
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 49.505 kb on + strand, within GFF2533 at 49.506 kb on - strand, within GFF2533 at 49.506 kb on - strand, within GFF2533 at 49.509 kb on + strand, within GFF2533 at 49.510 kb on - strand, within GFF2533 at 49.571 kb on - strand, within GFF2533 at 49.618 kb on + strand at 49.618 kb on + strand at 49.619 kb on - strand at 49.709 kb on - strand at 49.739 kb on + strand at 49.756 kb on - strand at 49.809 kb on + strand at 49.809 kb on + strand at 49.810 kb on - strand at 49.810 kb on - strand at 49.912 kb on - strand, within GFF2534 at 50.038 kb on + strand, within GFF2534 at 50.039 kb on - strand, within GFF2534 at 50.040 kb on + strand, within GFF2534 at 50.088 kb on - strand, within GFF2534 at 50.100 kb on + strand, within GFF2534 at 50.100 kb on + strand, within GFF2534 at 50.101 kb on - strand, within GFF2534 at 50.101 kb on - strand, within GFF2534 at 50.119 kb on - strand, within GFF2534 at 50.120 kb on + strand, within GFF2534 at 50.142 kb on + strand, within GFF2534 at 50.167 kb on - strand, within GFF2534 at 50.430 kb on - strand at 50.653 kb on + strand, within GFF2535 at 51.267 kb on + strand, within GFF2535 at 51.285 kb on + strand, within GFF2535 at 51.367 kb on - strand, within GFF2535 at 51.367 kb on - strand, within GFF2535 at 51.416 kb on + strand, within GFF2535 at 51.438 kb on + strand, within GFF2535 at 51.591 kb on - strand at 51.592 kb on + strand at 51.592 kb on + strand at 51.647 kb on - strand at 51.748 kb on - strand, within GFF2536 at 51.748 kb on - strand, within GFF2536 at 51.790 kb on + strand, within GFF2536 at 51.790 kb on + strand, within GFF2536 at 52.012 kb on - strand at 52.012 kb on - strand at 52.033 kb on - strand at 52.051 kb on + strand at 52.068 kb on - strand at 52.184 kb on - strand at 52.248 kb on + strand, within GFF2537 at 52.457 kb on + strand at 52.459 kb on + strand at 52.460 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 49,505 + GFF2533 0.83 +1.5 49,506 - GFF2533 0.83 -0.4 49,506 - GFF2533 0.83 +0.4 49,509 + GFF2533 0.83 +0.4 49,510 - GFF2533 0.83 +1.4 49,571 - GFF2533 0.87 -0.4 49,618 + -1.9 49,618 + -0.2 49,619 - +0.2 49,709 - -0.8 49,739 + -0.6 49,756 - +0.9 49,809 + +0.1 49,809 + -0.6 49,810 - +1.9 49,810 - -1.9 49,912 - GFF2534 0.21 +3.1 50,038 + GFF2534 0.47 +3.5 50,039 - GFF2534 0.47 +3.6 50,040 + GFF2534 0.47 +0.5 50,088 - GFF2534 0.57 +3.3 50,100 + GFF2534 0.59 +2.1 50,100 + GFF2534 0.59 +3.3 50,101 - GFF2534 0.60 +2.9 50,101 - GFF2534 0.60 +3.9 50,119 - GFF2534 0.63 +3.2 50,120 + GFF2534 0.63 +3.5 50,142 + GFF2534 0.68 +3.1 50,167 - GFF2534 0.73 +4.0 50,430 - -1.7 50,653 + GFF2535 0.15 -1.3 51,267 + GFF2535 0.72 +2.0 51,285 + GFF2535 0.74 +0.6 51,367 - GFF2535 0.81 +2.1 51,367 - GFF2535 0.81 +1.0 51,416 + GFF2535 0.86 -0.4 51,438 + GFF2535 0.88 -2.8 51,591 - +0.8 51,592 + -0.2 51,592 + +0.3 51,647 - +0.1 51,748 - GFF2536 0.32 +0.1 51,748 - GFF2536 0.32 +1.2 51,790 + GFF2536 0.43 +0.3 51,790 + GFF2536 0.43 -0.6 52,012 - -1.1 52,012 - -0.4 52,033 - +1.5 52,051 + +1.3 52,068 - -0.4 52,184 - +0.8 52,248 + GFF2537 0.39 -1.2 52,457 + -1.5 52,459 + -0.9 52,460 - +0.1
Or see this region's nucleotide sequence