Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF237 and GFF238 overlap by 4 nucleotides GFF238 and GFF239 overlap by 4 nucleotides GFF239 and GFF240 overlap by 1 nucleotides GFF240 and GFF241 are separated by 135 nucleotides
GFF237 - Diaminopimelate epimerase (EC 5.1.1.7), at 11,096 to 11,920
GFF237
GFF238 - Protein of unknown function DUF484, at 11,917 to 12,624
GFF238
GFF239 - Tyrosine recombinase XerC, at 12,621 to 13,523
GFF239
GFF240 - 'Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase' transl_table=11, at 13,523 to 14,239
GFF240
GFF241 - ATP-dependent DNA helicase UvrD/PcrA, at 14,375 to 16,537
GFF241
Position (kb) 12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 11.917 kb on - strand at 12.129 kb on + strand, within GFF238 at 12.330 kb on + strand, within GFF238 at 12.330 kb on + strand, within GFF238 at 12.620 kb on + strand at 13.346 kb on + strand, within GFF239 at 13.550 kb on - strand at 13.586 kb on + strand at 13.646 kb on + strand, within GFF240 at 13.647 kb on - strand, within GFF240 at 13.728 kb on - strand, within GFF240 at 13.972 kb on + strand, within GFF240 at 13.981 kb on - strand, within GFF240 at 14.027 kb on + strand, within GFF240 at 14.027 kb on + strand, within GFF240 at 14.027 kb on + strand, within GFF240 at 14.028 kb on - strand, within GFF240 at 14.028 kb on - strand, within GFF240 at 14.028 kb on - strand, within GFF240 at 14.028 kb on - strand, within GFF240 at 14.143 kb on + strand, within GFF240 at 14.236 kb on - strand at 14.238 kb on - strand at 14.307 kb on + strand at 14.311 kb on + strand at 14.388 kb on - strand at 14.404 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 11,917 - +2.9 12,129 + GFF238 0.30 -0.7 12,330 + GFF238 0.58 -3.2 12,330 + GFF238 0.58 -0.5 12,620 + +0.4 13,346 + GFF239 0.80 +0.3 13,550 - -0.4 13,586 + +0.5 13,646 + GFF240 0.17 -2.0 13,647 - GFF240 0.17 -0.8 13,728 - GFF240 0.29 -1.4 13,972 + GFF240 0.63 -0.9 13,981 - GFF240 0.64 -1.2 14,027 + GFF240 0.70 -1.7 14,027 + GFF240 0.70 -2.3 14,027 + GFF240 0.70 +0.4 14,028 - GFF240 0.70 -0.6 14,028 - GFF240 0.70 +0.1 14,028 - GFF240 0.70 +1.6 14,028 - GFF240 0.70 -0.3 14,143 + GFF240 0.86 -1.0 14,236 - -0.4 14,238 - +0.3 14,307 + +0.0 14,311 + +1.4 14,388 - +2.0 14,404 + -0.5
Or see this region's nucleotide sequence