Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2282 and GFF2283 are separated by 184 nucleotides GFF2283 and GFF2284 are separated by 203 nucleotides
GFF2282 - Cobalt-zinc-cadmium resistance protein, at 70,167 to 71,069
GFF2282
GFF2283 - 6-phosphofructokinase (EC 2.7.1.11), at 71,254 to 72,216
GFF2283
GFF2284 - Sulfate-binding protein Sbp, at 72,420 to 73,409
GFF2284
Position (kb) 71
72
73 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 70.263 kb on + strand, within GFF2282 at 70.281 kb on - strand, within GFF2282 at 70.428 kb on - strand, within GFF2282 at 70.469 kb on + strand, within GFF2282 at 70.551 kb on - strand, within GFF2282 at 70.553 kb on + strand, within GFF2282 at 70.554 kb on - strand, within GFF2282 at 70.554 kb on - strand, within GFF2282 at 70.582 kb on + strand, within GFF2282 at 70.617 kb on - strand, within GFF2282 at 70.740 kb on + strand, within GFF2282 at 70.742 kb on + strand, within GFF2282 at 70.751 kb on - strand, within GFF2282 at 70.751 kb on - strand, within GFF2282 at 70.762 kb on - strand, within GFF2282 at 70.780 kb on + strand, within GFF2282 at 71.009 kb on + strand at 71.009 kb on + strand at 71.010 kb on - strand at 71.068 kb on - strand at 71.164 kb on - strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.166 kb on + strand at 71.167 kb on - strand at 71.167 kb on - strand at 71.172 kb on + strand at 71.377 kb on + strand, within GFF2283 at 71.377 kb on + strand, within GFF2283 at 71.400 kb on + strand, within GFF2283 at 71.426 kb on + strand, within GFF2283 at 71.426 kb on + strand, within GFF2283 at 71.572 kb on + strand, within GFF2283 at 71.572 kb on + strand, within GFF2283 at 71.573 kb on - strand, within GFF2283 at 71.574 kb on + strand, within GFF2283 at 71.574 kb on + strand, within GFF2283 at 71.575 kb on - strand, within GFF2283 at 71.665 kb on - strand, within GFF2283 at 71.743 kb on - strand, within GFF2283 at 71.748 kb on + strand, within GFF2283 at 71.820 kb on + strand, within GFF2283 at 71.925 kb on + strand, within GFF2283 at 71.925 kb on + strand, within GFF2283 at 71.955 kb on + strand, within GFF2283 at 72.032 kb on - strand, within GFF2283 at 72.032 kb on - strand, within GFF2283 at 72.070 kb on - strand, within GFF2283 at 72.405 kb on + strand at 72.405 kb on + strand at 72.406 kb on - strand at 72.449 kb on - strand at 72.449 kb on - strand at 72.504 kb on + strand at 72.635 kb on + strand, within GFF2284 at 72.753 kb on + strand, within GFF2284 at 72.754 kb on - strand, within GFF2284 at 73.010 kb on + strand, within GFF2284 at 73.010 kb on + strand, within GFF2284 at 73.089 kb on - strand, within GFF2284 at 73.095 kb on - strand, within GFF2284 at 73.095 kb on - strand, within GFF2284 at 73.201 kb on - strand, within GFF2284
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 70,263 + GFF2282 0.11 +0.3 70,281 - GFF2282 0.13 -0.5 70,428 - GFF2282 0.29 +0.5 70,469 + GFF2282 0.33 -1.4 70,551 - GFF2282 0.43 +1.2 70,553 + GFF2282 0.43 -1.8 70,554 - GFF2282 0.43 +0.4 70,554 - GFF2282 0.43 +1.0 70,582 + GFF2282 0.46 -0.7 70,617 - GFF2282 0.50 +1.4 70,740 + GFF2282 0.63 -1.7 70,742 + GFF2282 0.64 -1.2 70,751 - GFF2282 0.65 +0.4 70,751 - GFF2282 0.65 +0.6 70,762 - GFF2282 0.66 +1.0 70,780 + GFF2282 0.68 -2.1 71,009 + -1.6 71,009 + -1.4 71,010 - +0.9 71,068 - +0.5 71,164 - +0.9 71,166 + -3.5 71,166 + -0.8 71,166 + -0.9 71,166 + -2.6 71,166 + -1.4 71,167 - +0.9 71,167 - +1.1 71,172 + -1.2 71,377 + GFF2283 0.13 +1.7 71,377 + GFF2283 0.13 +2.0 71,400 + GFF2283 0.15 +2.4 71,426 + GFF2283 0.18 +0.3 71,426 + GFF2283 0.18 +1.3 71,572 + GFF2283 0.33 +0.6 71,572 + GFF2283 0.33 -0.2 71,573 - GFF2283 0.33 -0.2 71,574 + GFF2283 0.33 -0.2 71,574 + GFF2283 0.33 +0.6 71,575 - GFF2283 0.33 -0.9 71,665 - GFF2283 0.43 +0.6 71,743 - GFF2283 0.51 +1.3 71,748 + GFF2283 0.51 +0.7 71,820 + GFF2283 0.59 +1.3 71,925 + GFF2283 0.70 +1.5 71,925 + GFF2283 0.70 +1.4 71,955 + GFF2283 0.73 +0.3 72,032 - GFF2283 0.81 +1.8 72,032 - GFF2283 0.81 +1.1 72,070 - GFF2283 0.85 +1.2 72,405 + -1.1 72,405 + -0.7 72,406 - +1.8 72,449 - +0.3 72,449 - -0.7 72,504 + -0.2 72,635 + GFF2284 0.22 +1.1 72,753 + GFF2284 0.34 -0.4 72,754 - GFF2284 0.34 -1.7 73,010 + GFF2284 0.60 -1.0 73,010 + GFF2284 0.60 -0.5 73,089 - GFF2284 0.68 -0.7 73,095 - GFF2284 0.68 -0.3 73,095 - GFF2284 0.68 -0.5 73,201 - GFF2284 0.79 +0.2
Or see this region's nucleotide sequence