Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF228

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF227 and GFF228 are separated by 21 nucleotidesGFF228 and GFF229 overlap by 4 nucleotides GFF227 - Homolog of E. coli HemX protein, at 1,955 to 3,124 GFF227 GFF228 - Uroporphyrinogen-III synthase (EC 4.2.1.75), at 3,146 to 3,886 GFF228 GFF229 - Porphobilinogen deaminase (EC 2.5.1.61), at 3,883 to 4,824 GFF229 Position (kb) 3 4Strain fitness (log2 ratio) -1 0 1 2 3 4at 2.176 kb on - strand, within GFF227at 2.176 kb on - strand, within GFF227at 2.289 kb on - strand, within GFF227at 2.292 kb on + strand, within GFF227at 2.293 kb on - strand, within GFF227at 2.354 kb on - strand, within GFF227at 2.573 kb on - strand, within GFF227at 2.753 kb on - strand, within GFF227at 2.823 kb on - strand, within GFF227at 2.971 kb on + strand, within GFF227at 3.011 kb on + strandat 3.011 kb on + strandat 3.147 kb on + strandat 3.147 kb on + strandat 3.153 kb on - strandat 4.860 kb on - strandat 4.869 kb on - strandat 4.878 kb on - strandat 4.878 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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2,176 - GFF227 0.19 +4.2
2,176 - GFF227 0.19 +2.8
2,289 - GFF227 0.29 +2.3
2,292 + GFF227 0.29 +2.1
2,293 - GFF227 0.29 +3.0
2,354 - GFF227 0.34 +2.9
2,573 - GFF227 0.53 +2.8
2,753 - GFF227 0.68 +2.8
2,823 - GFF227 0.74 +2.3
2,971 + GFF227 0.87 +3.4
3,011 + +3.0
3,011 + +3.7
3,147 + +2.1
3,147 + +4.3
3,153 - -0.3
4,860 - +1.4
4,869 - +1.1
4,878 - +0.2
4,878 - +1.5

Or see this region's nucleotide sequence