Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2246 and GFF2247 are separated by 193 nucleotides GFF2247 and GFF2248 overlap by 30 nucleotides GFF2248 and GFF2249 are separated by 0 nucleotides GFF2249 and GFF2250 are separated by 46 nucleotides GFF2250 and GFF2251 overlap by 4 nucleotides
GFF2246 - Lipase, GDXG family, at 39,413 to 40,342
GFF2246
GFF2247 - FIG01046414: hypothetical protein, at 40,536 to 40,769
GFF2247
GFF2248 - FIG00642734: hypothetical protein, at 40,740 to 40,871
GFF2248
GFF2249 - Putative merR family bacterial regulatory protein, at 40,872 to 41,162
GFF2249
GFF2250 - formate dehydrogenase formation protein FdhE, at 41,209 to 42,138
GFF2250
GFF2251 - Formate dehydrogenase O gamma subunit (EC 1.2.1.2), at 42,135 to 42,770
GFF2251
Position (kb) 40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 39.946 kb on + strand, within GFF2246 at 39.950 kb on - strand, within GFF2246 at 39.974 kb on - strand, within GFF2246 at 40.011 kb on + strand, within GFF2246 at 40.111 kb on - strand, within GFF2246 at 40.114 kb on - strand, within GFF2246 at 40.114 kb on - strand, within GFF2246 at 40.186 kb on + strand, within GFF2246 at 40.187 kb on - strand, within GFF2246 at 40.344 kb on + strand at 40.344 kb on + strand at 40.344 kb on + strand at 40.485 kb on - strand at 40.504 kb on - strand at 40.505 kb on + strand at 40.624 kb on - strand, within GFF2247 at 40.624 kb on - strand, within GFF2247 at 40.629 kb on - strand, within GFF2247 at 40.811 kb on + strand, within GFF2248 at 40.850 kb on + strand, within GFF2248 at 40.869 kb on + strand at 40.870 kb on - strand at 40.908 kb on - strand, within GFF2249 at 40.956 kb on - strand, within GFF2249 at 40.964 kb on + strand, within GFF2249 at 41.107 kb on + strand, within GFF2249 at 41.143 kb on + strand at 41.144 kb on - strand at 41.144 kb on - strand at 41.181 kb on + strand at 41.357 kb on + strand, within GFF2250 at 41.357 kb on + strand, within GFF2250 at 41.425 kb on + strand, within GFF2250 at 41.426 kb on - strand, within GFF2250 at 41.494 kb on - strand, within GFF2250 at 41.573 kb on + strand, within GFF2250 at 41.662 kb on - strand, within GFF2250 at 41.798 kb on - strand, within GFF2250 at 41.838 kb on + strand, within GFF2250 at 41.899 kb on - strand, within GFF2250 at 41.933 kb on + strand, within GFF2250 at 42.038 kb on + strand, within GFF2250 at 42.039 kb on - strand, within GFF2250 at 42.125 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 39,946 + GFF2246 0.57 +0.8 39,950 - GFF2246 0.58 -3.5 39,974 - GFF2246 0.60 +1.1 40,011 + GFF2246 0.64 +0.8 40,111 - GFF2246 0.75 -0.1 40,114 - GFF2246 0.75 -0.0 40,114 - GFF2246 0.75 -0.9 40,186 + GFF2246 0.83 +0.5 40,187 - GFF2246 0.83 +0.7 40,344 + -1.9 40,344 + +0.4 40,344 + -0.2 40,485 - +1.2 40,504 - -0.1 40,505 + +1.1 40,624 - GFF2247 0.38 -0.7 40,624 - GFF2247 0.38 -0.9 40,629 - GFF2247 0.40 +1.4 40,811 + GFF2248 0.54 -1.1 40,850 + GFF2248 0.83 -0.1 40,869 + -1.5 40,870 - +0.3 40,908 - GFF2249 0.12 -1.2 40,956 - GFF2249 0.29 -2.9 40,964 + GFF2249 0.32 -0.7 41,107 + GFF2249 0.81 -1.1 41,143 + -1.5 41,144 - +0.2 41,144 - +0.6 41,181 + +0.0 41,357 + GFF2250 0.16 -0.9 41,357 + GFF2250 0.16 +1.3 41,425 + GFF2250 0.23 +0.8 41,426 - GFF2250 0.23 -1.5 41,494 - GFF2250 0.31 +0.2 41,573 + GFF2250 0.39 +0.3 41,662 - GFF2250 0.49 -0.3 41,798 - GFF2250 0.63 +0.9 41,838 + GFF2250 0.68 +0.3 41,899 - GFF2250 0.74 +0.2 41,933 + GFF2250 0.78 +0.8 42,038 + GFF2250 0.89 +0.6 42,039 - GFF2250 0.89 -1.3 42,125 - -0.4
Or see this region's nucleotide sequence