Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2227

Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2226 and GFF2227 are separated by 1 nucleotidesGFF2227 and GFF2228 overlap by 8 nucleotides GFF2226 - Hypothetical radical SAM family enzyme in interesting gene cluster, at 19,780 to 21,021 GFF2226 GFF2227 - FIG01124638: hypothetical protein, at 21,023 to 21,898 GFF2227 GFF2228 - FIG00638667: hypothetical protein, at 21,891 to 22,877 GFF2228 Position (kb) 21 22Strain fitness (log2 ratio) -2 -1 0 1at 20.141 kb on + strand, within GFF2226at 20.304 kb on - strand, within GFF2226at 20.307 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.311 kb on - strand, within GFF2226at 20.338 kb on - strand, within GFF2226at 20.526 kb on + strand, within GFF2226at 20.527 kb on - strand, within GFF2226at 20.527 kb on - strand, within GFF2226at 20.531 kb on - strand, within GFF2226at 20.562 kb on + strand, within GFF2226at 20.716 kb on + strand, within GFF2226at 20.807 kb on + strand, within GFF2226at 20.886 kb on + strand, within GFF2226at 20.895 kb on - strand, within GFF2226at 20.895 kb on - strand, within GFF2226at 20.973 kb on + strandat 20.992 kb on - strandat 20.994 kb on - strandat 21.015 kb on + strandat 21.022 kb on - strandat 21.054 kb on + strandat 21.096 kb on + strandat 21.096 kb on + strandat 21.207 kb on + strand, within GFF2227at 21.207 kb on + strand, within GFF2227at 21.208 kb on - strand, within GFF2227at 21.303 kb on + strand, within GFF2227at 21.304 kb on - strand, within GFF2227at 21.388 kb on + strand, within GFF2227at 21.485 kb on - strand, within GFF2227at 21.625 kb on + strand, within GFF2227at 21.712 kb on - strand, within GFF2227at 21.787 kb on + strand, within GFF2227at 21.812 kb on - strandat 22.016 kb on + strand, within GFF2228at 22.111 kb on - strand, within GFF2228at 22.132 kb on + strand, within GFF2228at 22.153 kb on - strand, within GFF2228at 22.153 kb on - strand, within GFF2228at 22.305 kb on - strand, within GFF2228at 22.390 kb on + strand, within GFF2228at 22.678 kb on + strand, within GFF2228at 22.678 kb on + strand, within GFF2228at 22.678 kb on + strand, within GFF2228at 22.698 kb on + strand, within GFF2228at 22.726 kb on - strand, within GFF2228at 22.813 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
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20,141 + GFF2226 0.29 -0.6
20,304 - GFF2226 0.42 +0.5
20,307 - GFF2226 0.42 -0.2
20,311 - GFF2226 0.43 -0.2
20,311 - GFF2226 0.43 +0.8
20,311 - GFF2226 0.43 +0.4
20,338 - GFF2226 0.45 +0.3
20,526 + GFF2226 0.60 -0.0
20,527 - GFF2226 0.60 -0.6
20,527 - GFF2226 0.60 +0.8
20,531 - GFF2226 0.60 -2.3
20,562 + GFF2226 0.63 +0.5
20,716 + GFF2226 0.75 -1.0
20,807 + GFF2226 0.83 +0.3
20,886 + GFF2226 0.89 -0.3
20,895 - GFF2226 0.90 -1.1
20,895 - GFF2226 0.90 -0.3
20,973 + +0.1
20,992 - +0.5
20,994 - +1.2
21,015 + -1.0
21,022 - +0.0
21,054 + +1.0
21,096 + +0.7
21,096 + -0.8
21,207 + GFF2227 0.21 -0.7
21,207 + GFF2227 0.21 -1.6
21,208 - GFF2227 0.21 +0.2
21,303 + GFF2227 0.32 +0.0
21,304 - GFF2227 0.32 +0.4
21,388 + GFF2227 0.42 +0.2
21,485 - GFF2227 0.53 -0.4
21,625 + GFF2227 0.69 -0.3
21,712 - GFF2227 0.79 -0.1
21,787 + GFF2227 0.87 +0.2
21,812 - +0.6
22,016 + GFF2228 0.13 -0.7
22,111 - GFF2228 0.22 -1.2
22,132 + GFF2228 0.24 -0.1
22,153 - GFF2228 0.27 -0.1
22,153 - GFF2228 0.27 +0.1
22,305 - GFF2228 0.42 -0.6
22,390 + GFF2228 0.51 -0.4
22,678 + GFF2228 0.80 +0.1
22,678 + GFF2228 0.80 -1.4
22,678 + GFF2228 0.80 +0.0
22,698 + GFF2228 0.82 +0.0
22,726 - GFF2228 0.85 +0.6
22,813 - -1.9

Or see this region's nucleotide sequence