Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2170 and GFF2171 are separated by 234 nucleotides GFF2171 and GFF2172 are separated by 87 nucleotides GFF2172 and GFF2173 are separated by 70 nucleotides GFF2173 and GFF2174 are separated by 26 nucleotides
GFF2170 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 18,347 to 18,490
GFF2170
GFF2171 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 18,725 to 19,351
GFF2171
GFF2172 - Uracil permease, at 19,439 to 20,728
GFF2172
GFF2173 - Chromosomal replication initiator protein DnaA, at 20,799 to 21,524
GFF2173
GFF2174 - Arsenate reductase (EC 1.20.4.1), at 21,551 to 21,910
GFF2174
Position (kb) 19
20
21 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 18.639 kb on + strand at 18.640 kb on - strand at 18.799 kb on + strand, within GFF2171 at 18.828 kb on + strand, within GFF2171 at 19.029 kb on - strand, within GFF2171 at 19.061 kb on + strand, within GFF2171 at 19.177 kb on + strand, within GFF2171 at 19.186 kb on + strand, within GFF2171 at 19.260 kb on - strand, within GFF2171 at 19.299 kb on - strand at 19.499 kb on - strand at 19.558 kb on + strand at 19.697 kb on + strand, within GFF2172 at 20.016 kb on - strand, within GFF2172 at 20.300 kb on + strand, within GFF2172 at 20.301 kb on - strand, within GFF2172 at 20.301 kb on - strand, within GFF2172 at 20.462 kb on + strand, within GFF2172 at 20.691 kb on + strand at 20.774 kb on + strand at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.956 kb on - strand, within GFF2173 at 20.957 kb on + strand, within GFF2173 at 20.957 kb on + strand, within GFF2173 at 20.961 kb on + strand, within GFF2173 at 21.183 kb on + strand, within GFF2173 at 21.251 kb on - strand, within GFF2173
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 18,639 + +0.1 18,640 - -0.3 18,799 + GFF2171 0.12 +2.6 18,828 + GFF2171 0.16 +3.3 19,029 - GFF2171 0.48 -0.4 19,061 + GFF2171 0.54 +3.1 19,177 + GFF2171 0.72 +2.9 19,186 + GFF2171 0.74 +3.0 19,260 - GFF2171 0.85 +2.8 19,299 - +2.6 19,499 - -0.5 19,558 + +2.9 19,697 + GFF2172 0.20 +0.2 20,016 - GFF2172 0.45 -1.2 20,300 + GFF2172 0.67 -0.4 20,301 - GFF2172 0.67 -0.3 20,301 - GFF2172 0.67 -0.9 20,462 + GFF2172 0.79 +0.1 20,691 + -1.3 20,774 + +0.0 20,955 + GFF2173 0.21 +0.9 20,955 + GFF2173 0.21 -0.5 20,955 + GFF2173 0.21 -1.4 20,955 + GFF2173 0.21 -1.3 20,956 - GFF2173 0.22 +0.2 20,957 + GFF2173 0.22 +1.1 20,957 + GFF2173 0.22 +0.8 20,961 + GFF2173 0.22 -1.9 21,183 + GFF2173 0.53 +0.3 21,251 - GFF2173 0.62 -0.8
Or see this region's nucleotide sequence