Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2141 and GFF2142 are separated by 199 nucleotides GFF2142 and GFF2143 are separated by 7 nucleotides GFF2143 and GFF2144 are separated by 5 nucleotides
GFF2141 - LysR family transcriptional regulator QseA, at 32,692 to 33,603
GFF2141
GFF2142 - probable membrane protein YPO3684, at 33,803 to 34,006
GFF2142
GFF2143 - Membrane fusion component of tripartite multidrug resistance system, at 34,014 to 34,946
GFF2143
GFF2144 - FUSARIC ACID RESISTANCE PROTEIN FUSB / FUSARIC ACID RESISTANCE PROTEIN FUSC, at 34,952 to 36,919
GFF2144
Position (kb) 33
34
35 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 32.910 kb on - strand, within GFF2141 at 32.961 kb on + strand, within GFF2141 at 33.046 kb on - strand, within GFF2141 at 33.047 kb on + strand, within GFF2141 at 33.345 kb on + strand, within GFF2141 at 33.345 kb on + strand, within GFF2141 at 33.346 kb on - strand, within GFF2141 at 33.565 kb on - strand at 34.053 kb on - strand at 34.107 kb on + strand at 34.107 kb on + strand at 34.161 kb on - strand, within GFF2143 at 34.195 kb on + strand, within GFF2143 at 34.195 kb on + strand, within GFF2143 at 34.196 kb on - strand, within GFF2143 at 34.294 kb on + strand, within GFF2143 at 34.295 kb on - strand, within GFF2143 at 34.303 kb on - strand, within GFF2143 at 34.310 kb on - strand, within GFF2143 at 34.481 kb on + strand, within GFF2143 at 34.599 kb on + strand, within GFF2143 at 34.599 kb on + strand, within GFF2143 at 34.694 kb on + strand, within GFF2143 at 34.728 kb on - strand, within GFF2143 at 34.757 kb on - strand, within GFF2143
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 32,910 - GFF2141 0.24 -0.6 32,961 + GFF2141 0.29 -0.1 33,046 - GFF2141 0.39 -1.8 33,047 + GFF2141 0.39 +0.1 33,345 + GFF2141 0.72 -2.0 33,345 + GFF2141 0.72 -0.2 33,346 - GFF2141 0.72 -0.2 33,565 - +0.2 34,053 - -4.2 34,107 + +1.1 34,107 + -0.3 34,161 - GFF2143 0.16 -2.6 34,195 + GFF2143 0.19 -0.4 34,195 + GFF2143 0.19 +0.1 34,196 - GFF2143 0.20 -2.3 34,294 + GFF2143 0.30 +0.3 34,295 - GFF2143 0.30 -0.5 34,303 - GFF2143 0.31 -2.6 34,310 - GFF2143 0.32 -1.9 34,481 + GFF2143 0.50 -0.7 34,599 + GFF2143 0.63 +0.1 34,599 + GFF2143 0.63 -1.9 34,694 + GFF2143 0.73 -1.7 34,728 - GFF2143 0.77 -0.1 34,757 - GFF2143 0.80 +1.8
Or see this region's nucleotide sequence