Experiment: LB_plus_SM_buffer with FelixO1_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2093 and GFF2094 overlap by 4 nucleotides GFF2094 and GFF2095 are separated by 44 nucleotides GFF2095 and GFF2096 are separated by 114 nucleotides
GFF2093 - Alpha-ketoglutarate permease, at 1,008 to 2,309
GFF2093
GFF2094 - Putative outer membrane lipoprotein, at 2,306 to 2,629
GFF2094
GFF2095 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8), at 2,674 to 4,029
GFF2095
GFF2096 - Protein acetyltransferase, at 4,144 to 6,804
GFF2096
Position (kb)
2
3
4
5 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1.791 kb on - strand, within GFF2093 at 1.803 kb on + strand, within GFF2093 at 1.803 kb on + strand, within GFF2093 at 1.803 kb on + strand, within GFF2093 at 1.804 kb on - strand, within GFF2093 at 1.804 kb on - strand, within GFF2093 at 1.804 kb on - strand, within GFF2093 at 1.804 kb on - strand, within GFF2093 at 1.835 kb on + strand, within GFF2093 at 1.836 kb on - strand, within GFF2093 at 1.836 kb on - strand, within GFF2093 at 1.836 kb on - strand, within GFF2093 at 2.042 kb on - strand, within GFF2093 at 2.142 kb on + strand, within GFF2093 at 2.182 kb on - strand at 2.233 kb on - strand at 2.300 kb on - strand at 2.307 kb on + strand at 2.329 kb on + strand at 2.478 kb on - strand, within GFF2094 at 2.497 kb on - strand, within GFF2094 at 2.609 kb on - strand at 4.137 kb on - strand at 4.387 kb on + strand at 4.387 kb on + strand at 4.388 kb on - strand at 4.388 kb on - strand at 4.388 kb on - strand at 4.388 kb on - strand at 4.551 kb on - strand, within GFF2096
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage 0.5 MOI remove 1,791 - GFF2093 0.60 +1.9 1,803 + GFF2093 0.61 -0.9 1,803 + GFF2093 0.61 +0.3 1,803 + GFF2093 0.61 +2.1 1,804 - GFF2093 0.61 +0.6 1,804 - GFF2093 0.61 -0.2 1,804 - GFF2093 0.61 -1.0 1,804 - GFF2093 0.61 +0.5 1,835 + GFF2093 0.64 +1.2 1,836 - GFF2093 0.64 -0.4 1,836 - GFF2093 0.64 -0.1 1,836 - GFF2093 0.64 -0.3 2,042 - GFF2093 0.79 +0.5 2,142 + GFF2093 0.87 +0.4 2,182 - +0.6 2,233 - -0.6 2,300 - -0.2 2,307 + +0.2 2,329 + -1.7 2,478 - GFF2094 0.53 +0.4 2,497 - GFF2094 0.59 +0.7 2,609 - -0.6 4,137 - +0.6 4,387 + -1.1 4,387 + -0.7 4,388 - -0.3 4,388 - -0.5 4,388 - -2.6 4,388 - +0.0 4,551 - GFF2096 0.15 -2.7
Or see this region's nucleotide sequence